Next-generation sequencing (NGS) capture technologies have revolutionized genetic sequencing of ancient and degraded DNA.
By combining the scale of NGS with the focused power of hybridization-based target capture, sequencing efforts can highlight endogenous DNA regions that matter most, whether from host or microbial sources, and eliminate contaminating DNA from sources like exogenous bacteria and fungi. Together, these technologies have opened up genetic study to a wide range of plant, animal, and environmental sources that were previously out of reach, such as archaeological, paleoclimate, environmental, historical, museum, herbarium, and many more. Flexible, in-solution NGS capture with technology such as myBaits®, allows enrichment of even extremely rare, damaged, and short molecules (<50bp) that are impossible to assay with traditional PCR-based amplicon sequencing.
With myBaits® Capture Kits, targeted sequencing of even extinct animals for which a reference genome sequence is unavailable has become routine. Importantly, the adaptable nature of hybridization capture can tolerate unknown divergence between bait reference sequence and target molecules, which are frequently present in aDNA specimens due to both biological mutations as well as postmortem damage. myBaits® target capture solutions are perfect for any scale of genetic study; single loci such as mitochondrial DNA, hundreds to thousands of custom loci, and entire nuclear genomes.
The scientific team at Daicel Arbor Biosciences has extensive experience working with ancient DNA, and can offer detailed design and protocol advice to cater experiments to the unique features of degraded DNA samples. In addition, Daicel Arbor Biosciences offers in-house NGS services for ancient and degraded DNA samples in our dedicated anti-contamination facilities, where our team of experts utilizes the latest library preparation chemistry tailored for low-input, degraded nucleic acids.
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