Long-Insert Capture Technology and Long Read Sequencing
In addition to a long history of success with short-read sequencing platforms such as Illumina® , myBaits® kits can also enrich very long DNA molecules (e.g. 3-7 Kb) for sequencing on long-read platforms such as PacBio® and Oxford Nanopore®.
Long-read sequencing technologies can enable the resolution of structurally complex or repetitive genome regions that are unable to be resolved with short-read sequencing alone. Pairing the power of these long-read sequencing platforms with focused myBaits® target capture technology allows focus on specific regions of interest, without losing precious sequencing reads to non-critical genomic regions.
The scientific team at Daicel Arbor Biosciences can help design a custom panel that selects only the most specific hybridization sites to fully utilize the advantages of long insert technologies and thus maximize long-read sequencing efforts. Importantly, myBaits® panels are platform agnostic, so the exact same bait set used for long insert capture with PacBio or Oxford Nanopore technology can also be used to capture standard short-insert NGS libraries for sequencing on other platforms such as Illumina®, Ion Torrent®, or Genapsys®, increasing the accuracy and efficiency of genome mapping.
The active research community coupling hybridization capture and long-read sequencing have demonstrated the feasibility and utility of long-read sequencing for many challenging applications, such as sequencing of complex repetitive disease resistance genes in agriculturally important plant species. Review our growing list of featured publications below for a variety of examples of successful pairings of long-read sequencing and myBaits® Custom target capture kits.
Giolai, M. et al. (2017). Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire. BMC Genomics.
Giolai, M. et al. (2016). Targeted capture and sequencing of gene-sized DNA molecules.BioTechniques.
Steuernagel, B. et al. (2016). Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nature Biotechnology.
Witek, K. et al. (2016). Accelerated cloning of a potato late blight-resistance gene using RenSeq and SMRT sequencing. Nature Biotechnology.