myBaits Expert Human Affinities Manual [PDF]

The protocol and reagents provided with the myBaits Expert Human Affinities kit were developed for ancient DNA and other library types that are heavily degraded or contaminated with non-human DNA. It involves two rounds of hybridization capture, with hybridization incubated at high temperature (70°C), with hybrid bead-binding and washes performed at a lower temperature (55°C or room temperature). Each round involves an over-night incubation, and libraries are amplified following both rounds. When enriching non-contaminated/degraded libraries, a single round of enrichment is recommended.