myTags – FISH Probes
myTags Immortal
myTags® immortal probe libraries are amplifiable libraries that can be utilized to generate labeled myTags probes in-house.

Easily generate higher yields of labeled probes compared to other labeling methods while gaining the flexibility to select fluorophores or haptens of choice.  myTags immortal libraries are amplifiable, meaning there is an endless supply of probe library which can produce labeled probes from amplified pools of the original stock library. Immortal probe libraries are a great solution to minimizing long-term costs associated with large-scale projects. Additionally, we can provide oligo libraries with unique primer sets for labeling Oligopaints probes.

  • Flexible – Easily evaluate various probe labels
  • In-house Labeling – Product labeled probes as needed, when needed
  • Optimized Protocol – Generate high yields of labeled probes from low input amounts
  • Amplifiable – Product a never-ended supply of probes from the immortal library
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Contact our experts today to start your next (F)ISH project with myTags Immortal probes.

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The myTags labeling method and technical support provided by Daicel Arbor Biosciences delivers the confidence required to label your probes in-house. The labeling process involves two simple transcription processes to transcribe large amounts of single-stranded DNA probes from small amounts of input DNA.

If pre-labeled, ready-to-use probes are preferred, please review the myTags Labeled Library option for your application. Daicel Arbor Biosciences provides complimentary probe design for either immortal or labeled libraries in order to deliver the best performance possible.

Please contact us with any questions or determine which solution is best suited for your intended application.

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Design Process

myTags Design Process

Labeling Workflow

Do you have recommended protocols for labeling immortal libraries?

Yes, we provide the myTags labeling protocol with all myTags immortal libraries for performing the labeling process in your own lab.
What recommendations do you make for successful FISH assays?

Generally we recommend probe densities between 3-10 probes per kilobase for target regions larger than 50kb. For target regions between 10-50kb, probe densities should be on the higher end of that range, and we may recommend using multiple fluorophores per probe to boost the signal.
Do you provide design assistance for FISH probes?

We offer complementary FISH probe design service using our proprietary design algorithm for most types of FISH projects. Please contact us for design assistance.
Can I design my own FISH probe libraries to label using the myTags labeling protocol?

We can often accommodate customer-designed probes into the myTags labeling framework. Please contact us for recommendations on the design parameters and other information before designing your probe sequences.
Can you provide Oligopaints libraries?

Yes, we can synthesize immortal probe libraries that can be labeled using the Oligopaints labeling method. Please note these probe libraries are not compatible with the myTags labeling protocol due to sequence requirements of the Oligopaints method.
Can you design probes for unassembled genomes?

We can work with any sequenced data to design probes. Please contact us with a brief description of your project, including the name of your study species, genomic coordinates, and any additional information.
How quickly can you ship my order?

We generally ask for up to 2-3 weeks after an order is placed to ship myTags libraries.
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