The 16S rRNA gene sequence is frequently used to identify and profile membership of bacterial communities. The utility of studying 16S for microbial classification and phylogenetic analysis was demonstrated over 40 years ago by Carl Woese, and its impact has only increased over time. Defining and understanding the relationships within a microbial community has a broad range of important applications, including human health, environmental surveys, animal welfare, and agricultural improvements.
The myBaits® 16S-Hyb Panel enriches the entire gene, rather than just one or two hypervariable regions, leading to better taxonomic resolution and less bias than 16S amplicon sequencing. The panel can also efficiently identify rare taxa that may be contributing to novel phenotypes in conjunction with metagenomic studies, requiring less sequence depth for individual samples. Achieve improved characterization of microbial populations in metagenomic samples with a more cost-effective manner compared to shotgun sequencing alone, and more accurately than 16S amplicon sequencing, by combining 16S-Hyb rRNA gene enrichment with low-coverage shotgun sequencing.
- Convenient – Use the same NGS library preparation or protocol for shotgun sequencing
- Cost Effective – Identify rare taxa in metagenomic samples with less sequence depth
- Less Bias – Avoids issues associated with amplicon-based approaches
- Excellent Resolution – All constant and hypervariable regions are represented
- Open Platform – Compatible with any NGS library platform, including long-read sequencing
- Complete Solution – Kits include all necessary reagents to perform enrichment of NGS libraries