Custom Guide RNA Pools for Targeted NGS
Thanks to our ultra-efficient parallelized nucleic acid synthesis technology, Arbor is uniquely equipped to provide robust, affordable gRNA libraries of up to thousands of unique sequences, specifically for CRISPR-powered targeted enrichment or depletion in your species of choice. Contact us for a custom guide RNA pool powered by Arbor’s myNGS Guides™.
Like probe-based hybridization capture technology (e.g., myBaits®), the CRISPR/Cas system can be used for targeted high-throughput sequencing. In addition to standard targeted resequencing and rare variant detection, CRISPR/Cas-driven targeted sequencing is especially useful for resolving genomic regions too complex for short-molecule sequence capture. The several reported techniques are extremely versatile, and have been coupled with both short-read Illumina and long-read PacBio and Oxford Nanopore platforms.
CRISPR/Cas-driven targeted sequencing generally follows two basic procedural paradigms, enrichment or depletion. Enrichment techniques enable site-specific adapter ligation after sequestration from background molecules. Depletion techniques use the system’s site-specific nuclease activity to sever unwanted genomic sequences and render them non-sequenceable.
Whichever the application, the key component of all CRISPR/Cas-driven techniques is a collection (library) of sequence-targeting guide RNAs (gRNAs). When combined with Cas enzymes, these gRNA libraries drive complex sequence-specific effects in a simple, single reaction.
Thanks to our ultra-efficient parallelized nucleic acid synthesis technology, Arbor is uniquely equipped to provide robust, affordable, expert-designed gRNA libraries of up to thousands of unique sequences, perfect for CRISPR/Cas-driven targeted sequencing.