Oligos & Arrays
DNA Oligo Pools
Multiple applications utilize pools of single or double-stranded DNA oligos to assay a diverse array of sequences at once.

These applications include screening for promoter or protein sequences of a specific activity, shRNA gene knockdown, CRISPR-mediated genome editing, systematic evolution of ligands by exponential enrichment (SELEX), and oligo-selective sequencing (OS-seq). Guide RNA libraries for genome editing that target every gene in the human genome, for example, are a very powerful tool when they are cloned into a lentiviral vector system. The lentiviral particles can be used to infect cells with single hit kinetics where just one gene per cell is potentially inactivated. Following the lentiviral infection and selection through screening, the gene or genes involved in responding to a drug can be quickly uncovered.

After receiving an oligo pool, many researchers need to immediately clone the DNA oligos into a vector of their choice. This is best facilitated by receiving dsDNA that is either flanked on both ends with a restriction enzyme cut site or can be used directly in a variety of cloning methods, such as Golden Gate, Gibson and PCR assembly. We supply dsDNA oligos for any cloning procedure required.

All applications involving oligo libraries perform best when the supplied oligos have very high sequence fidelity. Low quality libraries, by comparison, can lead to false-positives or negatives that render data analysis nearly impossible. Daicel Arbor Biosciences utilizes best-in-class oligonucleotide chemistry combined with digital lithography for maximum flexibility in parallel oligo synthesis to deliver high-quality, low cost oligos to our clients. We have experience synthesizing millions of oligos simultaneously, and have developed a robust QC process to ensure delivery of the best oligo quality possible.

myLib – ssDNA and dsDNA Oligo Libraries
Custom oligonucleotide library synthesis utilizing Daicel Arbor Biosciences flexible oligo synthesis platform.

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