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iGEM
…and wiki (as applicable), including the product(s) used and a link to the company’s website (www.arborbiosci.com) Mention Daicel Arbor Biosciences and products on social media channels throughout the competition Place…
Effective Biophysical Modeling of Cell Free Transcription and Translation Processes
…composed of coupled ordinary differential equations, was tested by comparing simulations of two cell free synthetic circuits with experimental measurements generated in this study. First, we considered a simple circuit…
myBaits Expert Predesigned Panels
…taxonomic distances. Capture any molecule—Probe designs are compatible with both short- and long-read sequencing libraries built using virtually any library prep chemistry. Cost-effective—We produce these bait sets in high volume…
Next-generation sequencing data show rapid radiation and several long-distance dispersal events in early Costaceae
…fossils within Zingiberales, †Spirematospermum chandlerae and †Ensete oregonense, indicated a relatively recent diversification of Costaceae, between 18–9 Mya. Based on these data, the current pantropical distribution of the family is…
Cell-free transcription–translation: engineering biology from the nanometer to the millimeter scale (review)
Cell-free transcription–translation (TXTL) has become a highly versatile technology to construct, characterize and interrogate genetically programmed biomolecular systems implemented outside living organisms. By recapitulating gene expression in vitro, TXTL offers…
Variability in cell-free expression reactions can impact qualitative genetic circuit characterization
…genetic regulation elements, simple genetic circuit motifs, protein variants or metabolic pathways. However, variability in cell-free expression systems is a known challenge, whether between individuals, laboratories, instruments, or batches of…
Introduction to myTXTL cell-free protein expression
https://www.youtube.com/watch?v=-Vf7a0kgx-E This short video illustrates how the myTXTL system utilizes the power of cell-free expression for high throughput discovery in protein engineering….
The best of both worlds: Combining lineage-specific and universal bait sets in target-enrichment hybridization reactions
…(35–59%) targets resulted in a sizable reconstruction of loci for each target set (x̄ ≥ 70%). Conclusions High levels of enrichment and locus reconstruction for the two target sets demonstrate…
Targeting legume loci: A comparison of three methods for target enrichment bait design in Leguminosae phylogenomics
…and bait design, through validation, to tree inference. We compared three methods within Leguminosae (Fabaceae) and present a workflow for future efforts. Methods Using 30 transcriptomes, we compared Hyb-Seq, MarkerMiner,…
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