…single-stranded DNA (ssDNA) fluorescent probes through an intermediate RNA library. A double-stranded oligonucleotide library is amplified by transcription to create an RNA library. Next, dye- or hapten-conjugate primers are used…
…from both approaches for investigating microbial communities. These baits cover the diversity of all 16S rRNA sequences available in the Greengenes (v. 13.5) database, with no sequence having 400-fold) and…
…a range of samples. Reference: Burridge, A.J., Wilkinson, P.A., Winfield, M.O., Barker, G.L.A., Allen, A.M., Coghill, J.A., Waterfall, C., and Edwards, K.J. (2018). Conversion of array-based single nucleotide polymorphic markers…
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…variation, diverse habitats and different distribution patterns. Here, we used Hyb-Seq data to construct a well-resolved phylogeny of the C. zawadskii complex. Then, we performed comparative analyses of variation patterns…
…Jacob Enk, Ph.D. Director of Research, Development, and Applications Board members Ravindra Ramadhar President Joseph Barendt, Ph.D. Senior Director, International Business Development Daicel Life Sciences SBU Tatsushi Murakami President, CPI…
…in tissue collections. We extracted DNA from historical museum specimens and sequenced ultraconserved elements (UCEs) and mitochondrial DNA (mtDNA) to assess their phylogeny and divergence times. Phylogenies built from hundreds…
…capture for eDNA-based population studies. The mitochondrial target capture successfully retrieved 235x (90x-352x per base position) coverage of the whale shark mitogenome. Using a minor allele frequency of 5%, we…
…versus shotgun sequencing. Reference: Beaudry, M.S., J.C. Thomas, R.P. Baptista, A.H. Sullivan, W. Norfolk, A. Devault, J. Enk, T.J. Kieran, O.E. Rhodes, Jr., K.A. Perry-Dow, L.J. Rose, N.J. Bayona-Vásquez, A….
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