Yes, the Library Preparation Kit for myBaits is compatible with a wide range of input DNA qualities, from high-molecular weight to highly degraded. However, some degraded sample types (such as…
…(d)Cas9 from Streptococcus pyogenes targeting a GFP reporter gene. For complete information on the generation and use of this protocol, please refer to the paper by Marshall et al. (2018)….
…, Staphylococcus aureus , and Acinetobacter baumannii ) were grown in vitro for 3 days and then challenged with respective antibiotics (ciprofloxacin, daptomycin, and tigecycline) for an additional 24 h….
…from both approaches for investigating microbial communities. These baits cover the diversity of all 16S rRNA sequences available in the Greengenes (v. 13.5) database, with no sequence having 400-fold) and…
…in myTXTL Antibody/DS Master Mix and complexed with antigen prior to magnetic bead purification by His-tag (lane 1-2), Twin-Strep-tag™(lane 3), and Protein A (lane 4). Large scale ScFv expression and…
…least 8x depth of coverage).” (pg 21, Johnson, Pokorny, Dodsworth et al 2019, Syst Bio). Image is modified version of Figure 4 from Johnson, Pokorny, Dodsworth et al 2019, Syst…
…(POPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-poly(ethyleneglycol)-2000-N-[3-(2-pyridyldithio)propionate], so-called DSPE-PEG-PDP, at different molar ratios (respectively, 99.5/0.5, 97.5/2.5, and 95/5 mol %), and tBLM formation is characterized using QCM-D, SPR, and atomic force technology (AFM). We…
…and purified expression components. For paperlike materials, we found that (1) materials based on synthetic polymers were mostly incompatible with cell-free expression, (2) lysate-based reactions were largely insensitive to the…
…Illumina platform in the large and diverse angiosperm family Compositae (Asteraceae). The family is monophyletic, based on morphology and molecular data, yet many areas of the phylogeny have unresolved polytomies…
…the compartments. The system recreated oscillating protein expression patterns and protein gradients, and provides a stepping stone to creating “artificial cells” on a chip. Science, this issue p. 829 The…
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