We manufacture microarrays in a variety of formats with probe sequences typically up to 50-mer size. Below are details on designing probes, layout of probes and potential microarray formats on the slides. Please review prior to placing an order and contact us with any questions you may have about the process.
Either submit a list of probe sequences of your choice or request to use our complimentary design service in order to select high quality probe sequences. In either case, we will use proprietary software to screen all sequences and identify those that may be poorly synthesized, like long monotonous repeats, or generate cross-hybridization signals, like GT repeats in mammals. In the end, we will generate a list of robust probes with the highest specificity for their intended targets.
Choose how many replicates of your probe you would like to have on each array. While we usually recommend from 3 to 5 replicates, your experiment may call for a different number. You will also be able to choose if you want probe replicates randomly spread across the entire array area or if you prefer to have them grouped to form easily recognizable patterns.
Select from a variety of our standard microarray formats or request a custom format. Format selection is driven in part by the number of replicated probes on the array and by the hybridization method you plan to use, such as cover slip, Agilent cassettes, etc.