Like probe-based hybridization capture technology (e.g., myBaits®), the CRISPR/Cas system can be used for targeted high-throughput sequencing. In addition to standard targeted resequencing and rare variant detection, CRISPR/Cas-driven targeted sequencing is especially useful for resolving genomic regions too complex for short-molecule sequence capture. The several reported techniques are extremely versatile, and have been coupled with both short-read Illumina and long-read PacBio and Oxford Nanopore platforms.
Guide RNA Libraries
CRISPR/Cas-driven targeted sequencing generally follows two basic procedural paradigms, enrichment or depletion. Enrichment techniques enable site-specific adapter ligation after sequestration from background molecules. Depletion techniques use the system’s site-specific nuclease activity to sever unwanted genomic sequences and render them non-sequenceable.
Whichever the application, the key component of all CRISPR/Cas-driven techniques is a collection (library) of sequence-targeting guide RNAs (gRNAs). When combined with Cas enzymes, these gRNA libraries drive complex sequence-specific effects in a simple, single reaction.
Thanks to our ultra-efficient parallelized nucleic acid synthesis technology, Arbor is uniquely equipped to provide robust, affordable, expert-designed gRNA libraries of up to thousands of unique sequences, perfect for CRISPR/Cas-driven targeted sequencing. As with all of our genomics and synthetic biology products, we offer complimentary expert design consultation to help you achieve your goals.
For your next CRISPR/Cas-driven targeted sequencing project, contact us for a custom guide RNA library powered by Arbor’s myNGS Guides™
- Depletion of mitochondrial DNA (mtDNA) from ATAC-Seq libraries
- Removal of abundant unwanted sequences from RNA-seq libraries
- Isolation of long genomic fragments for downstream use
- Ultra-sensitive and accurate short fragment targeted sequencing
- Accurate targeted sequencing of repeat-rich regions
- Capture of gDNA and its associated proteins using the dCas9 mutant
As each application is unique, please contact us to discuss the specifics of myNGS Guides™ for your project.