myBaits® WGE – Whole Genome Enrichment

Whole Genome Enrichment (WGE) is target capture of genome-wide DNA from a complex DNA source.

Arbor Biosciences’ WGE bait manufacturing technology can cost-effectively produce biotinylated RNA baits representative of an entire nuclear genome. This allows for bulk enrichment of genome-wide endogenous DNA from complex metagenomic samples, such as environmental or ancient DNA. To manufacture custom WGE baits, all that is required is a sample of high-quality genomic DNA from an organism of interest, or a close relative, even if the genome has not been sequenced.

Recovering appreciable full-genome coverage from metagenomic, environmental, or ancient DNA samples with only shotgun sequencing data is likely to be both expensive and bioinformatically intense. Standard and custom WGE kits from Arbor Biosciences can help overcome these challenges. Discover how myBaits WGE kits can dramatically increase the percentage of endogenous DNA reads from an NGS dataset.

Due to the very deep requisite sequencing depths required to observe appreciable coverage of whole-genome data, especially from degraded or complex samples, Whole Genome Enrichment (WGE) kits are generally best suited for exploratory research projects. If the goal is to recover specific genes or markers of interest, then a focused custom myBaits panel is more appropriate for maximizing sequencing efficiency. If WGE is the proper application of choice, premade and custom WGE kits from Arbor Biosciences can help overcome many of these challenges.

Features & Benefits

Custom bait sets
Select from an organism of interest
Premade human baits
In stock and ready to ship for ancient DNA
Efficient genome-wide sequencing
Isolate genome of interest from complex samples
Cost-effective genomic bait manufacturing
Savings on baits and sequencing costs
Perfect for exploratory studies
Sequenced genome is not required

Figure 1. Schematic demonstrating the production and typical application of myBaits WGE kits. Baits can be produced from any pure eukaryotic or prokaryotic genomic DNA source, for example modern elephant. Arbor Biosciences’ unique process coverts this gDNA into a pool of biotinylated RNA baits. These baits can then enrich corresponding molecules from a user-supplied NGS library, for example from an ancient mammoth, via the process of myBaits in-solution hybridization capture. This enriched library allows for orders-of-magnitude more efficient NGS than would be possible using the full, non-enriched library.

Current Version: v4

For kits received starting in April 2018

Your kit has Arbor Biosciences packaging.


Previous Version: v3

For kits received prior to April 2018

Your kit has MYcroarray packaging.

Arbor Biosciences has stock genomic DNA (gDNA) from a variety of species, including Human (Caucasian Male & African Male). If your desired species gDNA is not available in our stock, please provide a pure, high-molecular weight gDNA sample, either lab-generated or from a commercial supplier. Arbor Biosciences will then utilize proprietary technology to transform the gDNA into ready-to-use genome-wide target capture baits.

Target capture necessarily requires subjecting your libraries to a bottleneck, wherein target molecules are captured and enriched, and non-target molecules are therefore removed. To have sufficient unique molecules for good sequencing coverage of your targets, successful captures DEPEND on the input of sufficiently complex libraries. For this reason, for libraries with a significant non-target component (e.g., ancient, forensic, or environmental samples), and especially for WGE captures with a very large full nuclear genome target size, we strongly recommend maximizing the target component in each capture by using as much input library as possible (up to 2 µg+), and consider two rounds of capture for higher percentage of reads on-target.

For best results, it is recommended that only amplified (non-PCR-free) NGS libraries are used for target capture. This provides multiple copies of each starting template molecule, increasing the chance of each individual molecule getting enriched. However if you need more starting material to reach the recommended amount, it is generally preferable to generate more library from fresh genomic DNA or a new batch of indexed library, rather than through extra amplification. This is because while some amplification is good, over-amplification risks reducing the observable complexity of your libraries through the uneven action of PCR bias, as some molecules will become relatively more abundant while others become rare. This is also true for manipulating your libraries after capture: amplify your post-capture libraries the minimum number of cycles necessary to reach the molarity required by your sequencing facility.

We generally do NOT recommend pooling multiple samples per capture reaction for very degraded and/or rare targets (e.g. ancient DNA), or for very large targets (e.g. a WGE baitset targeting a full nuclear genome). In fact, for highly degraded samples, you may achieve better results by performing multiple parallel WGE reactions per sample.

The myBaits WGE product ONLY offers bait production directly from high quality genomic DNA precursor material, which you must physically provide to us (either extracted in your lab or purchased via a third-party gDNA supplier). We do have the ability to design and synthesize synthetic RNA bait oligo pools via our standard myBaits custom kit product, however this is NOT an option for large nuclear genomes (e.g. from plants or animals), due to prohibitively large numbers of baits that would be required. However if you are working with an organism that has a smaller genome (e.g. from bacteria), or would be interested in capturing only a portion of an entire genome, then a custom myBaits kit may be possible. Please contact us with details about your project goals and budget, so we can discuss project options.

Ordering Information

Catalog # Description Quantity Price Contact Us
302416 myBaits WGE Custom 16 Reactions $1680 Order Now
302448 myBaits WGE Custom 48 Reactions $4560 Order Now
302496 myBaits WGE Custom 96 Reactions $8160 Order Now
302508 myBaits WGE Human 8 Reactions $840 Order Now
302548 myBaits WGE Human 48 Reactions $4560 Order Now
302596 myBaits WGE Human 96 Reactions $8160 Order Now

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