With more than a decade of experience providing effective tools for our life sciences customers, Daicel Arbor Biosciences is proud to offer a range of dynamic and innovative SARS-CoV-2 research tools for academic, industry, public health, and surveillance applications.
Tools for you
SARS-CoV-2 Research Solutions
Solutions for Every User
Research Applications
Viral Genomics for SARS-CoV-2 & FISH Probes
Viral genome sequences can be used to study viral evolution and phylogenetics, community transmission, non-human host natural reservoirs, and more. Rapidly reconstruct full or partial viral genome sequences from any sample type, even degraded or environmental, with the myBaits® Expert SARS-CoV-2 kit for NGS hybridization capture. Compared to amplicons or untargeted sequencing, hybridization capture is the most efficient genome sequencing method for novel variants and challenging samples, as it is the only targeted sequencing approach that is effective both with significant rearrangements or mutations and with target molecules of any size. Our myReads® NGS services scientists can also help you design a customized enrichment kit with even more targeted viral genomes, and/or perform the entire NGS workflow from RNA to sequencing.
Viral Dynamics for SARS-CoV-2
Directly visualize cellular locations of both native SARS-CoV-2 genomic, transcriptomic and/or antigenomic viral RNAs with the myTags® Expert SARS-CoV-2 suite of predesigned FISH probes, available in both ready-to-use and ready-to-label formats. The probe sets have been designed with high specificity for use in human cell lines and tissue, and are suitable for cell culture-based drug discovery and histological experiments. Our in-house FISH experts can provide information and advice on your experimental setup and design for best results.
Host Traits & Response
Comprehensively assess genome and transcriptome content at key loci in human or non-human host immune gene pathways and more, with myBaits Custom DNA-Seq or RNA-Seq kits for targeted next-generation sequencing via hybridization capture. We offer both kits as well as in-house NGS services to fit any project size and experimental need. Cytogenomics researchers can utilize our myTags custom FISH probes to directly visualize the cellular expression of key innate/adaptive human immune genes or other gene pathways of interest. Pair this with our myTags Expert SARS-CoV-2 probes for comprehensive cytogenetic profiling of host + viral behavior.
Drug Target Discovery & Viral Detection
Rapid development of effective solutions for the diagnosis and treatment of SARS-CoV-2 has been extremely critical in combating this current global pandemic. Cell-free protein expression technology enables researchers to accelerate design-build-test cycles which are key for any discovery process, reducing timelines from weeks to days. Our myTXTL® Cell-Free Expression platform can be utilized to either produce disease-specific antigens such as parts of the SPIKE protein or anti-viral effector molecules like ligand-binding proteins either from circular (plasmid) or linear DNA templates (gene fragments, PCR products). All TX-TL reagents are compatible with liquid handling systems supporting high-throughput sampling, and our synthetic biology experts can provide guidance for template design and technology implementation. In addition, researchers currently exploit sophisticated molecular switches in combination with cell-free expression systems for rapid and low-cost virus detection solutions.
Research Spotlight
SARS-CoV-2 Viral Genome Sequencing
Sequencing the complete SARS-CoV-2 viral genome allows for high-resolution genetic comparison between samples, which can help trace viral infection events. Tillett et al. (2021) report an apparent SARS-CoV-2 reinfection in an individual several months after an initial infection, based on detailed viral genome sequencing using the myBaits SARS-CoV-2 kit. The two infections had “genetically distinct” genome sequences, each with multiple differences versus the reference. This level of variation is indicative of two separate infection events, and supports the conclusion that achieving full natural immunity after SARS-CoV-2 infection may be more complicated than widely believed.
Read the Article: Tillett et al. (2021), The Lancet Infectious Diseases
Our Technologies
Targeted NGS with myBaits Hybridization Capture Kits
In-solution target capture, also known as target enrichment or sequence capture, utilizes pools of thousands of long custom biotinylated oligonucleotides to selectively enrich for specific regions of interest in a next-generation sequencing (NGS) library prior to sequencing. The ability to affordably manufacture complex pools of tens of thousands of different oligos, such as those powering Daicel Arbor’s myBaits hybridization capture kits, enables the feasibility of this technique for NGS projects of any scale.
Services for any NGS Application with myReads NGS Services
The NGS experts at Daicel Arbor Biosciences have collective decades of experience tackling library preparation, target capture, and next-generation sequencing for a wide variety of projects. We have optimized techniques for diverse and challenging sample types and applications, and will work with you to develop a project plan that meets your unique experimental needs. Our experts have successfully processed many thousands of samples representing a wide variety of organisms and sample types. Contact us today to learn how myReads NGS services can bring success to your next targeted sequencing project.
Cytogenetic Visualization with myTags Fish Probes
Fluorescent in-situ hybridization (FISH) provides direct visualization of viral activity in the cells or tissue of interest. Daicel Arbor’s myTags FISH probes are comprised of thousands of different synthetic oligos that are designed with optimized algorithms to hybridize only to their intended targets. Unlike alternative probe sources, these efficient probes provide bright and reliable target signals, while minimizing or eliminating background signals from repetitive regions and/or non-specific binding.
Protein Synthesis with myTXTL Cell-Free Expression Kits
In cell-free expression systems, gene transcription (TX) and translation (TL) is performed in a reaction tube upon mixing a DNA or RNA template with TX-TL reagents, thereby bypassing typical constraints of protein synthesis in vivo. The myTXTL technology from Daicel Arbor Biosciences is a versatile and easy-to-use E. coli cell-extract based protein production platform perfect for protein variant screenings, gene circuit prototyping, phage production and many more applications. Originally developed by Prof. Vincent Noireaux at the University of Minnesota, this revolutionary and powerful TX-TL technology has been further refined for superior robustness and to deliver high performance and reliable results.
Application Note
Pipeline for Comprehensive SARS-CoV-2 Genome Sequencing
Complete viral genome sequencing is critical for identifying mutational variants that will impact research in the fields of virology, immunology, epidemiology, and molecular evolution, among others. However, maximizing viral RNA sequencing reads from clinical specimens or laboratory samples can be challenging, particularly due to the amount of host background RNA. Here, we present a targeted NGS strategy that pairs the Swift Biosciences RNA Library Kit with the Daicel Arbor Biosciences myBaits Expert SARS-CoV-2 kit with excellent enrichment sensitivity and specificity.
Daicel Arbor Biosciences products are for research use only.
