Cell-Free Protein Expression

In cell-free protein expression systems, gene transcription (TX) and translation (TL) is unbound from constraints required for cell culture based processes. The myTXTL^® systems utilizes the endogenous TX-TL machinery derived from E. coli where gene expression is initialized by simply adding a nucleotide template, DNA or RNA, to the master mix and detecting recombinant proteins after only a few minutes. Due to the lack of cell walls and the independency from cell growth and viability, cell-free systems allow rapid and parallel production of soluble or toxic proteins and other complex biological structures, like bacteriophages, under open-reaction conditions. This enables easy manipulation, addition of chaperone molecules and straightforward downstream processing of synthesized proteins, enzymes and phages.

The myTXTL^® technology from Daicel Arbor Biosciences is a versatile and easy-to-use cell-free expression platform for protein synthesis, enzyme engineering, and synthetic biology processes in industry and academia. Based on a system originally developed by Prof. Vincent Noireaux at the University of Minnesota, this revolutionary and powerful TX-TL technology has been further refined at Daicel Arbor Biosciences toward even greater robustness and to deliver reliable, consistent performance.

myTXTL^® has been employed for various applications in protein engineering and synthetic biology research. Whether starting from circular or linear DNA templates, Daicel Arbor Biosciences provides a solution to meet your application. Pairing an automated liquid handler with a cell-free TX-TL system is ideal for rapid iteration of design-build-test cycles when screening libraries, analyzing metabolic pathways or testing gene circuits that will be later placed in a cell-based system.