Synthetic Biology
Cell-Free Protein Expression
Daicel Arbor Biosciences provides flexible solutions for protein engineering and synthetic biology applications.

In cell-free protein expression systems, gene transcription (TX) and translation (TL) is unbound from constraints required for cell culture based processes. The myTXTL® systems utilizes the endogenous TX-TL machinery derived from E. coli where gene expression is initialized by simply adding a nucleotide template, DNA or RNA, to the master mix and detecting recombinant proteins after only a few minutes. Due to the lack of cell walls and the independency from cell growth and viability, cell-free systems allow rapid and parallel production of soluble or toxic proteins and other complex biological structures, like bacteriophages, under open-reaction conditions. This enables easy manipulation, addition of chaperone molecules and straightforward downstream processing of synthesized proteins, enzymes and phages.

The myTXTL® technology from Daicel Arbor Biosciences is a versatile and easy-to-use cell-free expression platform for protein synthesis, enzyme engineering, and synthetic biology processes in industry and academia. Based on a system originally developed by Prof. Vincent Noireaux at the University of Minnesota, this revolutionary and powerful TX-TL technology has been further refined at Daicel Arbor Biosciences toward even greater robustness and to deliver reliable, consistent performance.

myTXTL® has been employed for various applications in protein engineering and synthetic biology research. Whether starting from circular or linear DNA templates, Daicel Arbor Biosciences provides a solution to meet your application. Pairing an automated liquid handler with a cell-free TX-TL system is ideal for rapid iteration of design-build-test cycles when screening libraries, analyzing metabolic pathways or testing gene circuits that will be later placed in a cell-based system.



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Upcoming Webinar
Interested in Cell-Free DNA Methylation in Cancer Biomarker Discovery and Validation?
See our News section on the home page for more information on the July 29 webinar presented by Dr. Bodour Salhia,
USC Keck School of Medicine.

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