myTXTL – Cell-Free Expression
myTXTL Toolbox 2.0 Plasmid Collection
The myTXTL Toolbox 2.0 Plasmid Collection is an excellent source for designing comprehensive gene circuits.

The combination of synthetic gene networks with in vitro protein production technology opens new innovative fields of application in medicine and biotechnology. Examples are environmental sensors, controls for biomanufacturing of biofuels, stem cell medicine, gene therapy (CRISPR), and manufacturing of functional materials. Cell-free platforms help to overcome limitations occurring in living host organisms, which are typically tied to the biochemical cross-talk between the host cell and the artificial gene circuit and a consequence of the potentially cytotoxic effect of the exogeneous gene network on the living cell on the one hand, and the availability of resources for transcription and translation, DNA replication and metabolites on the other. As those resources are dependent on the cell density, growth rate and cultivation conditions, deploying cell-free systems for gene circuit testing creates a more controlled environment thus increasing reproducibility and robustness of the circuit behavior and output.

The myTXTL® Toolbox 2.0 Plasmid that you receive upon ordering is intended for propagation purposes only. To make the plasmid suitable for in vitro expression in myTXTL, please follow our recommended 2-step procedure to generate high-purity plasmid samples. For propagation of P70a-vector, please also read our Tech note about “Preparation of chemo-competent KL740 cells for amplification of P70a vectors“.

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Ordering Information
Cat#
Qty
Price
pTXTL-P19a-S24
P19a-S24
502005
$65.00
pTXTL-P19a-S28
P19a-S28
502007
$65.00
pTXTL-P19a-S28-ssrA
P19a-S28-ssrA
502008
$65.00
pTXTL-P19a-S38
P19a-S38
502009
$65.00
pTXTL-P19a-deCFP
P19a-deCFP
502002
$65.00
pTXTL-P19a-deGFP
P19a-deGFP
502001
$65.00
pTXTL-P19a-deYFP
P19a-deYFP
502003
$65.00
pTXTL-P19a-ntrC
P19a-ntrC
502004
$65.00
pTXTL-P19a-ompA-S24
P19a-ompA-S24
502006
$65.00
pTXTL-P24a-S28
P24a-S28
502011
$65.00
pTXTL-P24a-S28-ssrA
P24a-S28-ssrA
502013
$65.00
pTXTL-P24a-S38
P24a-S38
502014
$65.00
pTXTL-P24a-deGFP
P24a-deGFP
502010
$65.00
pTXTL-P24a-ompA-S38
P24a-ompA-S38
502012
$65.00
pTXTL-P28a-aH
P28a-aH
502015
$65.00
pTXTL-P28a-aH-eGFP
P28a-aH-eGFP
502018
$65.00
pTXTL-P28a-cI-ssrA
P28a-cI-ssrA
502017
$65.00
pTXTL-P28a-S19
P28a-S19
502031
$65.00
pTXTL-P28a-S19-ssA
P28a-S19-ssA
502032
$65.00
pTXTL-P28a-S24-ssrA
P28a-S24-ssrA
502033
$65.00
pTXTL-P28a-S38
P28a-S38
502035
$65.00
pTXTL-P28a-S54
P28a-S54
502036
$65.00
pTXTL-P28a-T7rnap
pTXTL-P28a-T7rnap
502118
$65.00
pTXTL-P28a-deGFP
P28a-deGFP
502019
$65.00
pTXTL-P28a-deGFP-ssrA
P28a-deGFP-ssrA
502020
$65.00
pTXTL-P28a-deYFP
P28a-deYFP
502021
$65.00
pTXTL-P28a-flgM
P28a-flgM
502022
$65.00
pTXTL-P28a-flgM-ssrA
P28a-flgM-ssrA
502023
$65.00
pTXTL-P28a-mApple
P28a-mApple
502025
$65.00
pTXTL-P28a-mazE-ssrA
P28a-mazE-ssrA
502027
$65.00
pTXTL-P28a-mreC
P28a-mreC
502029
$65.00
pTXTL-P28a-mreB
P28a-mreB
502028
$65.00
pTXTL-P28a-ntrC
P28a-ntrC
502030
$65.00
pTXTL-P28a-ompA-S24-ssrA
P28a-ompA-S24-ssrA
502034
$65.00
pTXTL-P28a-tetO1-deGFP-ssrA
pTXTL-P28a-tetO1-deGFP-ssrA
502132
$65.00
pTXTL-P28a-tetR
P28a-tetR
502037
$65.00
pTXTL-P28a-tetR-ssrA
pTXTL-P28a-tetR-ssrA
502131
$65.00
pTXTL-P28a-venus-mreB
P28a-venus-mreB
502038
$65.00
pTXTL-P28a-venus-mreB-18L
P28a-venus-mreB-18L
502039
$65.00
pTXTL-P32a-deGFP
P32a-deGFP
502040
$65.00
pTXTL-P38a-S19
P38a-S19
502043
$65.00
pTXTL-P38a-S19-ssrA
P38a-S19-ssrA
502044
$65.00
pTXTL-P38a-S28
P38a-S28
502045
$65.00
pTXTL-P38a-S28-ssrA
P38a-S28-ssrA
502046
$65.00
pTXTL-P38a-S54
P38a-S54
502047
$65.00
pTXTL-P38a-deGFP
P38a-deGFP
502041
$65.00
pTXTL-P38a-ntrC
P38a-ntrC
502042
$65.00
pTXTL-P54a-S19
P54a-S19
502049
$65.00
pTXTL-P54a-S24
P54a-S24
502050
$65.00
pTXTL-P54a-S28
P54a-S28
502051
$65.00
pTXTL-P54a-S38
P54a-S38
502052
$65.00
pTXTL-P54a-deGFP
P54a-deGFP
502048
$65.00
pTXTL-P70a-broccoli
pTXTL-P70a-broccoli
502119
$65.00
pTXTL-P70a-cI
pTXTL-P70a-cI
502120
$65.00
pTXTL-P70a-cI-ssrA
P70a-cI-ssrA
502054
$65.00
pTXTL-P70a-clpXP
P70a-clpXP
502053
$65.00
pTXTL-P70a-deCFP
P70a-deCFP
502055
$65.00
pTXTL-P70a-S19
P70a-S19
502067
$65.00
pTXTL-P70a-S19-ssrA
P70a-S19-ssrA
502068
$65.00
pTXTL-P70a-S24
P70a-S24
502069
$65.00
pTXTL-P70a-S28
P70a-S28
502072
$65.00
pTXTL-P70a-S28-ssrA
P70a-S28-ssrA
502073
$65.00
pTXTL-P70a-S28-ybaQ
P70a-S28-ybaQ
502074
$65.00
pTXTL-P70a-dTomato
P70a-dTomato
502060
$65.00
pTXTL-P70a-deGFP
P70a-deGFP
502056
$65.00
pTXTL-P70a-deGFP-ssrA
P70a-deGFP-ssrA
502057
$65.00
pTXTL-P70a-deYFP
P70a-deYFP
502058
$65.00
pTXTL-P70a-dmVenus
P70a-dmVenus
502059
$65.00
pTXTL-P70a-luc
pTXTL-P70a-luc
502121
$65.00
pTXTL-P70a-mApple
P70a-mApple
502061
$65.00
pTXTL-P70a-mGapt-deGFP
P70a-mGapt-deGFP
502065
$65.00
pTXTL-P70a-mGapt1
P70a-mGapt1
502064
$65.00
pTXTL-P70a-mRuby
P70a-mRuby
502066
$65.00
pTXTL-P70a-mmCherry
P70a-mmCherry
502063
$65.00
pTXTL-P70a-ntrC
P70a-ntrC
502071
$65.00
pTXTL-P70a-ompA-S24
P70a-ompA-S24
502070
$65.00
pTXTL-P70a-ompA-S38
P70a-ompA-S38
502078
$65.00
pTXTL-P70a-S32
P70a-S32
502075
$65.00
pTXTL-P70a-S32-ssrA
P70a-S32-ssrA
502076
$65.00
pTXTL-P70a-S38
P70a-S38
502077
$65.00
pTXTL-P70a-T3rnap
P70a-T3rnap
502081
$65.00
pTXTL-P70a-S54
P70a-S54
502079
$65.00
pTXTL-P70a-T7rnap
P70a-T7rnap
502082
$65.00
pTXTL-P70a-U3-deGFP
P70a-U3-deGFP
502083
$65.00
pTXTL-P70a-U4-deGFP
P70a-U4-deGFP
502084
$65.00
pTXTL-P70a-U5-deGFP
P70a-U5-deGFP
502085
$65.00
pTXTL-P70a-tagRFPT1
P70a-tagRFPT1
502080
$65.00
pTXTL-P70a-venus
P70a-venus
502086
$65.00
pTXTL-P70b-U3-deGFP
P70b-U3-deGFP
502090
$65.00
pTXTL-P70b-U4-deGFP
P70b-U4-deGFP
502091
$65.00
pTXTL-P70b-broccoli
pTXTL-P70b-broccoli
502122
$65.00
pTXTL-P70b-deGFP
P70b-deGFP
502087
$65.00
pTXTL-P70c-deGFP
P70c-deGFP
502092
$65.00
pTXTL-P70b-mGapt1
P70b-mGapt1
502088
$65.00
pTXTL-P70c-U4-deGFP
P70c-U4-deGFP
502096
$65.00
pTXTL-P70c-U3-deGFP
P70c-U3-deGFP
502095
$65.00
pTXTL-P70c-mGapt1
P70c-mGapt1
502093
$65.00
pTXTL-P70d-deGFP
P70d-deGFP
502097
$65.00
pTXTL-P70e-broccoli
pTXTL-P70e-broccoli
502123
$65.00
pTXTL-P70e-deGFP
pTXTL-P70e-deGFP
502124
$65.00
pTXTL-PLlacO1-deCFP
pTXTL-PLlacO1-deCFP
502126
$65.00
pTXTL-PLlacO1-deGFP
pTXTL-PLlacO1-deGFP
502125
$65.00
pTXTL-PLlacO1-tetR
pTXTL-PLlacO1-tetR
502127
$65.00
pTXTL-PLtetO1-deGFP
PLtetO1-deGFP
502098
$65.00
pTXTL-PLtetO1-deGFP-ssrA
PLtetO1-deGFP-ssrA
502099
$65.00
pTXTL-PLtetO1-deGFP-ybaQ
PLtetO1-deGFP-ybaQ
502100
$65.00
pTXTL-PLtetO1-lacO1
pTXTL-PLtetO1-lacO1
502128
$65.00
pTXTL-PLtetO1-mmCherry-ssrA
pTXTL-PLtetO1-mmCherry-ssrA
502133
$65.00
pTXTL-PLtetO1-tetR
pTXTL-PLtetO1-tetR
502129
$65.00
pTXTL-T3p-deGFP
T3p-deGFP
502104
$65.00
pTXTL-T7p11-deGFP
T7p11-deGFP
502109
$65.00
pTXTL-T7p11-mGapt
T7p11-mGapt
502110
$65.00
pTXTL-pC-mGapt
pC-mGapt
502102
$65.00
pTXTL-pCa-deGFP
pCa-deGFP
502103
$65.00
pTXTL-T7p14-aH
T7p14-aH
502113
$65.00
pTXTL-T7p14-aH-eGFP
T7p14-aH-eGFP
502114
$65.00
pTXTL-T7p14-deGFP
T7p14-deGFP
502111
$65.00
pTXTL-T7p14-luc
pTXTL-T7p14-luc
502130
$65.00
pTXTL-T7p16-deGFP
T7p16-deGFP
502115
$65.00
pTXTL-T7p14-mGapt
T7p14-mGapt
502112
$65.00
pTXTL-T7p16-mGapt
T7p16-mGapt
502116
$65.00
pTXTL-T7p7-deGFP
T7p7-deGFP
502105
$65.00
pTXTL-T7p7-mGapt
T7p7-mGapt
502106
$65.00
pTXTL-T7p8-deGFP
T7p8-deGFP
502107
$65.00
pTXTL-T7p8-mGapt
T7p8-mGapt
502108
$65.00
Available and in-stock for immediate shipment.
For Propagation Only

Please note: The plasmid sample you receive upon ordering is intended for propagation purposes only. To make the plasmid suitable for in vitro expression in myTXTL, follow the recommended 2-step procedure to generate high-purity plasmid samples. For propagation of P70a-vector, please also read the Tech Note about “Preparation of chemo-competent KL740 cells for amplification of P70a vectors“.



Overview

Gene Circuits

The myTXTL Toolbox 2.0 Plasmid Collection was designed and intensively studied by Vincent Noireaux, PhD at the University of Minnesota and co-workers. It contains over one hundred plasmids with various promoters and open reading frames (ORFs) to investigate gene regulation and molecule turnover. Available open reading frames include a wide selection of transcription factors, TXTL modulators and fluorescent reporter proteins to build multi-stage gene circuits.

A gene circuit executed in any myTXTL® kit is required to start with a σ70-specific promoter, as gene expression in myTXTL® relies entirely on the endogenous TXTL machinery of E. coli. Its modular and pre-designed format makes it also ideal for educational purposes to train students and demonstrate basic concepts of synthetic biology.

Please note: The plasmid sample you receive upon ordering is intended for propagation purposes only. To make the plasmid suitable for in vitro expression in myTXTL, follow the recommended 2-step procedure to generate high-purity plasmid samples. For propagation of P70a-vector, please also read the Tech Note about “Preparation of chemo-competent KL740 cells for amplification of P70a vectors“.

Performance

Fig 1. Utilizing a 2-Gene Transcriptional Cascade. In this example a Sigma 70 promoter is driving the expression of T7 RNA Polymerase which in-turn drives the expression of deGFP on another plasmid with a T7 promoter.

Fig 2. Co-expression Required to Drive a Reporter. In this circuit, expression of deGFP requires both phosphorylation of Nitrogen regulator protein C (NtrC) as well as presence of Sigma 54.

Fig 3. Effect of plasmid concentration on in vitro protein production. deGFP expression is regulated by the interaction of the endogenous E. coli core RNA polymerase and the primary sigma factor 70 (σ70) with the σ70-specific promoter P70a.

Fig 4. Protein synthesis in myTXTL using the T7 expression system. deGFP expression under the control of the bacteriophage T7 promoter (PT7) is facilitated by initial expression of T7 RNA polymerase from an additional plasmid.

Publications

Featured Publications

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