LOCUS       T7_Fab_Trastuzumab__Linear_DNA        1881 bp    DNA     linear   UNA 24-JUN-2024
DEFINITION  .
ACCESSION   urn.local...aq-hvrkkx1
VERSION     urn.local...aq-hvrkkx1
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /locus_tag="T7 Promoter"
                     /ApEinfo_label="T7 Promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T7 Promoter"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NdeI Site"
     ORF             131..811
                     /locus_tag="Light Chain"
                     /ApEinfo_label="Light Chain"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="Light Chain_6XHis"
     motif           814..842
                     /created_by="User"
                     /label="UTR1"
     motif           847..852
                     /created_by="User"
                     /label="NdeI Site"
     gene            850..1635
                     /locus_tag="Heavy Chain"
                     /ApEinfo_label="Heavy Chain"
                     /ApEinfo_fwdcolor="#e1031e"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="Heavy Chain-TwinStrep"
     motif           1552..1575
                     /created_by="User"
                     /label="StrepTag"
     motif           1576..1611
                     /created_by="User"
                     /modified_by="User"
                     /label="Linker"
     motif           1612..1635
                     /created_by="User"
                     /label="StrepTag"
     motif           1639..1644
                     /created_by="User"
                     /label="XhoI"
     terminator      1692..1781
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(1867..1881)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggatattc agatgaccca gagcccgagc agcctgagcg cgagcgtggg
      181 cgatcgcgtg accattacct gccgcgcgag ccaggatgtg aacaccgcgg tggcgtggta
      241 tcagcagaaa ccgggcaaag cgccgaaact gctgatttat agcgcgagct ttctgtatag
      301 cggcgtgccg agccgcttta gcggcagccg cagcggcacc gattttaccc tgaccattag
      361 cagcctgcag ccggaagatt ttgcgaccta ttattgccag cagcattata ccaccccgcc
      421 gacctttggc cagggcacca aagtggaaat taaacgcacc gtggcggcgc cgagcgtgtt
      481 tatttttccg ccgagcgatg aacagctgaa aagcggcacc gcgagcgtgg tgtgcctgct
      541 gaacaacttt tatccgcgcg aagcgaaagt gcagtggaaa gtggataacg cgctgcagag
      601 cggcaacagc caggaaagcg tgaccgaaca ggatagcaaa gatagcacct atagcctgag
      661 cagcaccctg accctgagca aagcggatta tgaaaaacat aaagtgtatg cgtgcgaagt
      721 gacccatcag ggcctgagca gcccggtgac caaaagcttt aaccgcggcg aatgcggcgg
      781 cggcggcagc catcaccatc accatcacta agaaataatt ttgtttaact ttaagaagga
      841 gatatacata tggaagtgca gctggtggaa agcggcggcg gcctggtgca gccgggcggc
      901 agcctgcgcc tgagctgcgc ggcgagcggc tttaacatta aagataccta tattcattgg
      961 gtgcgccagg cgccgggcaa aggcctggaa tgggtggcgc gcatttatcc gaccaacggc
     1021 tatacccgct atgcggatag cgtgaaaggc cgctttacca ttagcgcgga taccagcaaa
     1081 aacaccgcgt atctgcagat gaacagcctg cgcgcggaag ataccgcggt gtattattgc
     1141 agccgctggg gcggcgatgg cttttatgcg atggattatt ggggccaggg caccctggtg
     1201 accgtgagca gcgcgagcac caaaggcccg agcgtgtttc cgctggcgcc gagcagcaaa
     1261 agcaccagcg gcggcaccgc ggcgctgggc tgcctggtga aagattattt tccggaaccg
     1321 gtgaccgtga gctggaacag cggcgcgctg accagcggcg tgcatacctt tccggcggtg
     1381 ctgcagagca gcggcctgta tagcctgagc agcgtggtga ccgtgccgag cagcagcctg
     1441 ggcacccaga cctatatttg caacgtgaac cataaaccga gcaacaccaa agtggataaa
     1501 aaagtggaac cgaaaagctg cgataaaacc cataccggcg gcggcggcag ctggagccat
     1561 ccgcagtttg aaaaaggcgg cggcagcggc ggcggcagcg gcggcggcag ctggagccat
     1621 ccgcagtttg aaaaataact cgagccttag gagatccggc tgctaacaaa gcccgaaagg
     1681 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1741 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg
     1801 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     1861 gggcggcggc caaagcggtc g
//