LOCUS T7_Fab_Trastuzumab_Plasmid 4968 bp ds-DNA circular 26-JUN-2024 DEFINITION . ACCESSION urn.local...g-hvqzy67 KEYWORDS . SOURCE ORGANISM . . COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers primer_bind 1..16 /created_by="User" /Sequence="CGGCCACGATGCGTCC" /label="T7LPfor PRIMER" promoter 51..69 /locus_tag="T7 Promoter" /ApEinfo_label="T7 Promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="T7 Promoter" 5'UTR 95..123 /created_by="User" /label="UTR1" motif 128..133 /created_by="User" /label="NdeI Site" ORF 131..811 /locus_tag="Light Chain" /ApEinfo_label="Light Chain" /ApEinfo_fwdcolor="#ff797d" /ApEinfo_revcolor="#ff102f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /modified_by="User" /label="Light Chain_6XHis" motif 814..842 /created_by="User" /label="UTR1" motif 847..852 /created_by="User" /label="NdeI Site" ORF 850..1635 /locus_tag="Heavy Chain" /ApEinfo_label="Heavy Chain" /ApEinfo_fwdcolor="#e1031e" /ApEinfo_revcolor="#ff102f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /modified_by="User" /label="Heavy Chain-TwinStrep" motif 1552..1575 /created_by="User" /label="StrepTag" motif 1576..1611 /created_by="User" /modified_by="User" /label="Linker" motif 1612..1635 /created_by="User" /label="StrepTag" motif 1639..1644 /created_by="User" /label="XhoI" terminator 1692..1781 /created_by="User" /label="T7 Terminator" misc_feature complement(1867..1881) /created_by="User" /Sequence="CGACCGCTTTGGCCG" /label="T7LPrev PRIMER" /note="Geneious type: primer_bind_reverse" rep_origin complement(2562..3244) /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /modified_by="User" /label="ColE1 Origin" ORF complement(3342..4199) /Imported_from="Pla sMapper" /Transferred_From="amp marker" /Transferred_Similarity="99.88%" /modified_by="User" /label="AmpR" promoter complement(4241..4269) /Imported_from="Pla sMapper" /Transferred_From="amp prom" /Transferred_Similarity="100.00%" /modified_by="User" /label="Amp Promoter" ORIGIN 1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact 61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg 121 agatatacat atggatattc agatgaccca gagcccgagc agcctgagcg cgagcgtggg 181 cgatcgcgtg accattacct gccgcgcgag ccaggatgtg aacaccgcgg tggcgtggta 241 tcagcagaaa ccgggcaaag cgccgaaact gctgatttat agcgcgagct ttctgtatag 301 cggcgtgccg agccgcttta gcggcagccg cagcggcacc gattttaccc tgaccattag 361 cagcctgcag ccggaagatt ttgcgaccta ttattgccag cagcattata ccaccccgcc 421 gacctttggc cagggcacca aagtggaaat taaacgcacc gtggcggcgc cgagcgtgtt 481 tatttttccg ccgagcgatg aacagctgaa aagcggcacc gcgagcgtgg tgtgcctgct 541 gaacaacttt tatccgcgcg aagcgaaagt gcagtggaaa gtggataacg cgctgcagag 601 cggcaacagc caggaaagcg tgaccgaaca ggatagcaaa gatagcacct atagcctgag 661 cagcaccctg accctgagca aagcggatta tgaaaaacat aaagtgtatg cgtgcgaagt 721 gacccatcag ggcctgagca gcccggtgac caaaagcttt aaccgcggcg aatgcggcgg 781 cggcggcagc catcaccatc accatcacta agaaataatt ttgtttaact ttaagaagga 841 gatatacata tggaagtgca gctggtggaa agcggcggcg gcctggtgca gccgggcggc 901 agcctgcgcc tgagctgcgc ggcgagcggc tttaacatta aagataccta tattcattgg 961 gtgcgccagg cgccgggcaa aggcctggaa tgggtggcgc gcatttatcc gaccaacggc 1021 tatacccgct atgcggatag cgtgaaaggc cgctttacca ttagcgcgga taccagcaaa 1081 aacaccgcgt atctgcagat gaacagcctg cgcgcggaag ataccgcggt gtattattgc 1141 agccgctggg gcggcgatgg cttttatgcg atggattatt ggggccaggg caccctggtg 1201 accgtgagca gcgcgagcac caaaggcccg agcgtgtttc cgctggcgcc gagcagcaaa 1261 agcaccagcg gcggcaccgc ggcgctgggc tgcctggtga aagattattt tccggaaccg 1321 gtgaccgtga gctggaacag cggcgcgctg accagcggcg tgcatacctt tccggcggtg 1381 ctgcagagca gcggcctgta tagcctgagc agcgtggtga ccgtgccgag cagcagcctg 1441 ggcacccaga cctatatttg caacgtgaac cataaaccga gcaacaccaa agtggataaa 1501 aaagtggaac cgaaaagctg cgataaaacc cataccggcg gcggcggcag ctggagccat 1561 ccgcagtttg aaaaaggcgg cggcagcggc ggcggcagcg gcggcggcag ctggagccat 1621 ccgcagtttg aaaaataact cgagccttag gagatccggc tgctaacaaa gcccgaaagg 1681 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta 1741 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg 1801 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact 1861 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc 1921 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata 1981 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg 2041 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt 2101 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca 2161 tgagaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 2221 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 2281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 2341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 2401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 2461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 2521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 2581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 2641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 2701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 2761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 2821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 2881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 2941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 3001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 3061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 3121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 3181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 3241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 3301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 3361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 3421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 3481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 3541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 3601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 3661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 3721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 3781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 3841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 3901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 3961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 4021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 4081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 4141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 4201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 4261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 4321 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 4381 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 4441 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 4501 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 4561 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga 4621 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 4681 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 4741 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 4801 agcttgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac 4861 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga 4921 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgc //