LOCUS       T7_Fab_Trastuzumab_Plasmid        4968 bp ds-DNA     circular     26-JUN-2024
DEFINITION  .
ACCESSION   urn.local...g-hvqzy67
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /locus_tag="T7 Promoter"
                     /ApEinfo_label="T7 Promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T7 Promoter"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NdeI Site"
     ORF             131..811
                     /locus_tag="Light Chain"
                     /ApEinfo_label="Light Chain"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="Light Chain_6XHis"
     motif           814..842
                     /created_by="User"
                     /label="UTR1"
     motif           847..852
                     /created_by="User"
                     /label="NdeI Site"
     ORF             850..1635
                     /locus_tag="Heavy Chain"
                     /ApEinfo_label="Heavy Chain"
                     /ApEinfo_fwdcolor="#e1031e"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="Heavy Chain-TwinStrep"
     motif           1552..1575
                     /created_by="User"
                     /label="StrepTag"
     motif           1576..1611
                     /created_by="User"
                     /modified_by="User"
                     /label="Linker"
     motif           1612..1635
                     /created_by="User"
                     /label="StrepTag"
     motif           1639..1644
                     /created_by="User"
                     /label="XhoI"
     terminator      1692..1781
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(1867..1881)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(2562..3244)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.52%"
                     /modified_by="User"
                     /label="ColE1 Origin"
     ORF             complement(3342..4199)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /modified_by="User"
                     /label="AmpR"
     promoter        complement(4241..4269)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="Amp Promoter"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggatattc agatgaccca gagcccgagc agcctgagcg cgagcgtggg
      181 cgatcgcgtg accattacct gccgcgcgag ccaggatgtg aacaccgcgg tggcgtggta
      241 tcagcagaaa ccgggcaaag cgccgaaact gctgatttat agcgcgagct ttctgtatag
      301 cggcgtgccg agccgcttta gcggcagccg cagcggcacc gattttaccc tgaccattag
      361 cagcctgcag ccggaagatt ttgcgaccta ttattgccag cagcattata ccaccccgcc
      421 gacctttggc cagggcacca aagtggaaat taaacgcacc gtggcggcgc cgagcgtgtt
      481 tatttttccg ccgagcgatg aacagctgaa aagcggcacc gcgagcgtgg tgtgcctgct
      541 gaacaacttt tatccgcgcg aagcgaaagt gcagtggaaa gtggataacg cgctgcagag
      601 cggcaacagc caggaaagcg tgaccgaaca ggatagcaaa gatagcacct atagcctgag
      661 cagcaccctg accctgagca aagcggatta tgaaaaacat aaagtgtatg cgtgcgaagt
      721 gacccatcag ggcctgagca gcccggtgac caaaagcttt aaccgcggcg aatgcggcgg
      781 cggcggcagc catcaccatc accatcacta agaaataatt ttgtttaact ttaagaagga
      841 gatatacata tggaagtgca gctggtggaa agcggcggcg gcctggtgca gccgggcggc
      901 agcctgcgcc tgagctgcgc ggcgagcggc tttaacatta aagataccta tattcattgg
      961 gtgcgccagg cgccgggcaa aggcctggaa tgggtggcgc gcatttatcc gaccaacggc
     1021 tatacccgct atgcggatag cgtgaaaggc cgctttacca ttagcgcgga taccagcaaa
     1081 aacaccgcgt atctgcagat gaacagcctg cgcgcggaag ataccgcggt gtattattgc
     1141 agccgctggg gcggcgatgg cttttatgcg atggattatt ggggccaggg caccctggtg
     1201 accgtgagca gcgcgagcac caaaggcccg agcgtgtttc cgctggcgcc gagcagcaaa
     1261 agcaccagcg gcggcaccgc ggcgctgggc tgcctggtga aagattattt tccggaaccg
     1321 gtgaccgtga gctggaacag cggcgcgctg accagcggcg tgcatacctt tccggcggtg
     1381 ctgcagagca gcggcctgta tagcctgagc agcgtggtga ccgtgccgag cagcagcctg
     1441 ggcacccaga cctatatttg caacgtgaac cataaaccga gcaacaccaa agtggataaa
     1501 aaagtggaac cgaaaagctg cgataaaacc cataccggcg gcggcggcag ctggagccat
     1561 ccgcagtttg aaaaaggcgg cggcagcggc ggcggcagcg gcggcggcag ctggagccat
     1621 ccgcagtttg aaaaataact cgagccttag gagatccggc tgctaacaaa gcccgaaagg
     1681 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1741 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg
     1801 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     1861 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     1921 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     1981 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     2041 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     2101 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
     2161 tgagaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2221 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4321 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     4381 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     4441 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     4501 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     4561 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4621 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4681 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     4741 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     4801 agcttgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     4861 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     4921 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgc
//
