LOCUS       T7_GLuc_Control_Linear_DNA         960 bp    DNA     linear   UNA 24-JUN-2024
DEFINITION  .
ACCESSION   urn.local...b2-hvrkpw2
VERSION     urn.local...b2-hvrkpw2
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..70
                     /created_by="User"
                     /label="T7 Promoter"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     5'UTR           128..133
                     /created_by="User"
                     /label="NcoI Site"
     ORF             130..714
                     /created_by="User"
                     /label="GLucDura_8XHis"
     motif           718..723
                     /created_by="User"
                     /label="XhoI Site"
     terminator      771..860
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(946..960)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tgggagttaa agtattattt gctctaatat gtatcgcggt ggccgaagcg
      181 aaaccgaccg aaaacaacga agatttcaac atcgtggctg tggcgtcgaa ttttgcgacc
      241 accgatttag atgcggatcg tggcaaactg ccgggcaaaa agctgccgct ggaggttctg
      301 aaggagctgg aggcgaacgc ccgtaaagct ggctgcaccc gtggttgtct tatctgcctg
      361 tctcacatta agtgcacgcc gaaaatgaaa aagttcatcc cgggtcgctg ccatacctac
      421 gaaggtgata aggagagcgc acaaggtggt attggtgaag cgattgtgga catcccggaa
      481 attccgggct ttaaagactt ggagccgctg gagcagttta tcgcccaagt tgacctctgc
      541 gttgactgta ctacgggttg cttgaagggc ctggcaaatg ttcagtgtag cgatttgctg
      601 aagaagtggc tgccacagcg ctgcgctacc ttcgcgtcca aaatccaagg tcaggtcgac
      661 aaaattaagg gcgcaggtgg cgacggcagc caccatcatc atcaccacca ccactaactc
      721 gagccttagg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      781 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      841 tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg
      901 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
//
