LOCUS       T7_GLuc_Control_Plasmid        4047 bp    DNA     circular UNA 24-JUN-2024
DEFINITION  .
ACCESSION   urn.local...1c-hvr25ww
VERSION     urn.local...1c-hvr25ww
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        51..70
                     /created_by="User"
                     /label="T7 Promoter"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     5'UTR           128..133
                     /created_by="User"
                     /label="NcoI Site"
     ORF             130..714
                     /created_by="User"
                     /label="GLucDura_8XHis"
     motif           718..723
                     /created_by="User"
                     /label="XhoI Site"
     terminator      771..860
                     /created_by="User"
                     /label="T7 Terminator"
     rep_origin      1641..2323
                     /created_by="User"
                     /label="ColE1 Origin"
     ORF             complement(2421..3278)
                     /created_by="User"
                     /modified_by="User"
                     /label="AmpR"
     promoter        complement(3320..3348)
                     /created_by="User"
                     /label="Amp Promoter"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tgggagttaa agtattattt gctctaatat gtatcgcggt ggccgaagcg
      181 aaaccgaccg aaaacaacga agatttcaac atcgtggctg tggcgtcgaa ttttgcgacc
      241 accgatttag atgcggatcg tggcaaactg ccgggcaaaa agctgccgct ggaggttctg
      301 aaggagctgg aggcgaacgc ccgtaaagct ggctgcaccc gtggttgtct tatctgcctg
      361 tctcacatta agtgcacgcc gaaaatgaaa aagttcatcc cgggtcgctg ccatacctac
      421 gaaggtgata aggagagcgc acaaggtggt attggtgaag cgattgtgga catcccggaa
      481 attccgggct ttaaagactt ggagccgctg gagcagttta tcgcccaagt tgacctctgc
      541 gttgactgta ctacgggttg cttgaagggc ctggcaaatg ttcagtgtag cgatttgctg
      601 aagaagtggc tgccacagcg ctgcgctacc ttcgcgtcca aaatccaagg tcaggtcgac
      661 aaaattaagg gcgcaggtgg cgacggcagc caccatcatc atcaccacca ccactaactc
      721 gagccttagg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      781 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      841 tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg
      901 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
      961 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
     1021 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     1081 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     1141 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     1201 ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattcgt aatcatgtca
     1261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3421 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     3481 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     3541 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     3601 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3661 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     3721 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     3781 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     3841 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc aaggagatgg
     3901 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     3961 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     4021 caaccgcacc tgtggcgccg gtgatgc
//
