LOCUS       T7_ScFv_Trastuzumab_6XHis_Linear_DNA        1138 bp    SYN     linear   UNA 24-JUN-2024
DEFINITION  .
ACCESSION   urn.local...ba-hvrky5g
VERSION     urn.local...ba-hvrky5g
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     motif           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NdeI Site"
     CDS             131..892
                     /modified_by="User"
                     /label="ScFv-Tratuzumab_6XHis"
     motif           896..901
                     /created_by="User"
                     /label="XhoI Site"
     terminator      949..1038
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(1124..1138)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggatatac aaatgacaca gagtccctca tccttgtcgg cgtccgtggg
      181 cgacagagtc acgatcacct gccgcgcgag tcaggatgtt aataccgcgg tggcctggta
      241 tcagcaaaaa ccgggcaagg caccgaaact gctcatttac agcgcgtctt ttctgtatag
      301 cggtgttccg tcccgcttca gcggtagccg tagcggcacc gattttaccc ttaccatcag
      361 cagcttgcaa ccagaggact tcgccaccta ttactgccag cagcattaca ccacccctcc
      421 gacctttggt caaggtacga aggtggagat caagggctcc tcgtctggtg gtggctccgg
      481 cgggggcagt ggcggtggag gctctagcga agttcaatta gtggagagcg gtggcggtct
      541 ggtgcaaccg ggtggctccc tgcgtctgtc ttgtgcagcg tcaggtttca acattaaaga
      601 tacctacatt cattgggttc gtcaggcacc gggtaagggt ttggaatggg ttgcgcgtat
      661 ttacccgacg aacggttata cgcgttatgc tgacagcgtg aaaggccgtt tcaccatcag
      721 cgcggacacc agcaaaaaca ctgcgtatct gcagatgaat agcctgcgtg ctgaagacac
      781 tgccgtttac tactgcagcc gctggggtgg cgatggtttt tacgctatgg attactgggg
      841 tcaggggacc ctggtcactg taagctctgg ctcccatcac caccaccacc actaactcga
      901 gccttaggag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      961 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     1021 ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta
     1081 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcg
//
