LOCUS       T7_ScFv_Trastuzumab_6XHis_Plasmid        4225 bp    SYN     circular     26-JUN-2024
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     .
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     motif           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NdeI Site"
     CDS             131..892
                     /modified_by="User"
                     /label="ScFv-Tratuzumab_6XHis"
     motif           896..901
                     /created_by="User"
                     /label="XhoI Site"
     terminator      949..1038
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(1124..1138)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      1819..2501
                     /created_by="User"
                     /label="ColE1 Origin"
     CDS             complement(2596..3456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     promoter        complement(3498..3526)
                     /created_by="User"
                     /label="Amp Promoter"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggatatac aaatgacaca gagtccctca tccttgtcgg cgtccgtggg
      181 cgacagagtc acgatcacct gccgcgcgag tcaggatgtt aataccgcgg tggcctggta
      241 tcagcaaaaa ccgggcaagg caccgaaact gctcatttac agcgcgtctt ttctgtatag
      301 cggtgttccg tcccgcttca gcggtagccg tagcggcacc gattttaccc ttaccatcag
      361 cagcttgcaa ccagaggact tcgccaccta ttactgccag cagcattaca ccacccctcc
      421 gacctttggt caaggtacga aggtggagat caagggctcc tcgtctggtg gtggctccgg
      481 cgggggcagt ggcggtggag gctctagcga agttcaatta gtggagagcg gtggcggtct
      541 ggtgcaaccg ggtggctccc tgcgtctgtc ttgtgcagcg tcaggtttca acattaaaga
      601 tacctacatt cattgggttc gtcaggcacc gggtaagggt ttggaatggg ttgcgcgtat
      661 ttacccgacg aacggttata cgcgttatgc tgacagcgtg aaaggccgtt tcaccatcag
      721 cgcggacacc agcaaaaaca ctgcgtatct gcagatgaat agcctgcgtg ctgaagacac
      781 tgccgtttac tactgcagcc gctggggtgg cgatggtttt tacgctatgg attactgggg
      841 tcaggggacc ctggtcactg taagctctgg ctcccatcac caccaccacc actaactcga
      901 gccttaggag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      961 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     1021 ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta
     1081 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga
     1141 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac
     1201 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac
     1261 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
     1321 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
     1381 accgcattaa agcttatcga tgataagctg tcaaacatga gaattcgtaa tcatgtcata
     1441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     3601 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     3661 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     3721 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     3781 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     3841 tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     3901 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     3961 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     4021 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcaa ggagatggcg
     4081 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     4141 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     4201 accgcacctg tggcgccggt gatgc
//
