LOCUS       T7_Trastuzumab_VH_TwinStrep_Linear_DNA        1828 bp    DNA     linear   UNA 24-JUN-2024
DEFINITION  .
ACCESSION   urn.local...bl-hvrl19v
VERSION     urn.local...bl-hvrl19v
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NdeI Site"
     motif           131..1498
                     /created_by="User"
                     /modified_by="User"
                     /label="Trastuzumab-VH-TS"
     motif           1499..1522
                     /created_by="User"
                     /label="TwinStrepTag"
     motif           1523..1558
                     /created_by="User"
                     /label="Linker"
     motif           1559..1582
                     /created_by="User"
                     /label="TwinStrepTag"
     motif           1586..1591
                     /created_by="User"
                     /label="XhoI Site"
     terminator      1639..1728
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(1814..1828)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggaagtgc agctggtgga aagcggcggc ggcctggtgc agccgggcgg
      181 cagcctgcgc ctgagctgcg cggcgagcgg ctttaacatt aaagatacct atattcattg
      241 ggtgcgccag gcgccgggca aaggcctgga atgggtggcg cgcatttatc cgaccaacgg
      301 ctatacccgc tatgcggata gcgtgaaagg ccgctttacc attagcgcgg ataccagcaa
      361 aaacaccgcg tatctgcaga tgaacagcct gcgcgcggaa gataccgcgg tgtattattg
      421 cagccgctgg ggcggcgatg gcttttatgc gatggattat tggggccagg gcaccctggt
      481 gaccgtgagc agcgcgagca ccaaaggccc gagcgtgttt ccgctggcgc cgagcagcaa
      541 aagcaccagc ggcggcaccg cggcgctggg ctgcctggtg aaagattatt ttccggaacc
      601 ggtgaccgtg agctggaaca gcggcgcgct gaccagcggc gtgcatacct ttccggcggt
      661 gctgcagagc agcggcctgt atagcctgag cagcgtggtg accgtgccga gcagcagcct
      721 gggcacccag acctatattt gcaacgtgaa ccataaaccg agcaacacca aagtggataa
      781 aaaagtggaa ccgaaaagct gcgataaaac ccatacctgc ccgccgtgcc cggcgccgga
      841 actgctgggc ggcccgagcg tgtttctgtt tccgccgaaa ccgaaagata ccctgatgat
      901 tagccgcacc ccggaagtga cctgcgtggt ggtggatgtg agccatgaag atccggaagt
      961 gaaatttaac tggtatgtgg atggcgtgga agtgcataac gcgaaaacca aaccgcgcga
     1021 agaacagtat aacagcacct atcgcgtggt gagcgtgctg accgtgctgc atcaggattg
     1081 gctgaacggc aaagaatata aatgcaaagt gagcaacaaa gcgctgccgg cgccgattga
     1141 aaaaaccatt agcaaagcga aaggccagcc gcgcgaaccg caggtgtata ccctgccgcc
     1201 gagccgcgaa gaaatgacca aaaaccaggt gagcctgacc tgcctggtga aaggctttta
     1261 tccgagcgat attgcggtgg aatgggaaag caacggccag ccggaaaaca actataaaac
     1321 caccccgccg gtgctggata gcgatggcag cttttttctg tatagcaaac tgaccgtgga
     1381 taaaagccgc tggcagcagg gcaacgtgtt tagctgcagc gtgatgcatg aagcgctgca
     1441 taaccattat acccagaaaa gcctgagcct gagcccgggc aaaggcggcg gcggcagctg
     1501 gagccatccg cagtttgaaa aaggcggcgg cagcggcggc ggcagcggcg gcggcagctg
     1561 gagccatccg cagtttgaaa aataactcga gccttaggag atccggctgc taacaaagcc
     1621 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     1681 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca
     1741 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     1801 caggactggg cggcggccaa agcggtcg
//
