LOCUS       T7_Trastuzumab_VH_TwinStrep_Plasmid        4915 bp ds-DNA     circular     26-JUN-2024
DEFINITION  .
ACCESSION   urn.local...h-hvqzy67
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NdeI Site"
     motif           131..1498
                     /created_by="User"
                     /modified_by="User"
                     /label="Trastuzumab-VH-TS"
     motif           1499..1522
                     /created_by="User"
                     /label="TwinStrepTag"
     motif           1523..1558
                     /created_by="User"
                     /label="Linker"
     motif           1559..1582
                     /created_by="User"
                     /label="TwinStrepTag"
     motif           1586..1591
                     /created_by="User"
                     /label="XhoI Site"
     terminator      1639..1728
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(1814..1828)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      2509..3191
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.52%"
                     /modified_by="User"
                     /label="ColE1 Origin"
     ORF             complement(3289..4146)
                     /created_by="User"
                     /label="AmpR"
     promoter        complement(4188..4216)
                     /created_by="User"
                     /label="Amp Promoter"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggaagtgc agctggtgga aagcggcggc ggcctggtgc agccgggcgg
      181 cagcctgcgc ctgagctgcg cggcgagcgg ctttaacatt aaagatacct atattcattg
      241 ggtgcgccag gcgccgggca aaggcctgga atgggtggcg cgcatttatc cgaccaacgg
      301 ctatacccgc tatgcggata gcgtgaaagg ccgctttacc attagcgcgg ataccagcaa
      361 aaacaccgcg tatctgcaga tgaacagcct gcgcgcggaa gataccgcgg tgtattattg
      421 cagccgctgg ggcggcgatg gcttttatgc gatggattat tggggccagg gcaccctggt
      481 gaccgtgagc agcgcgagca ccaaaggccc gagcgtgttt ccgctggcgc cgagcagcaa
      541 aagcaccagc ggcggcaccg cggcgctggg ctgcctggtg aaagattatt ttccggaacc
      601 ggtgaccgtg agctggaaca gcggcgcgct gaccagcggc gtgcatacct ttccggcggt
      661 gctgcagagc agcggcctgt atagcctgag cagcgtggtg accgtgccga gcagcagcct
      721 gggcacccag acctatattt gcaacgtgaa ccataaaccg agcaacacca aagtggataa
      781 aaaagtggaa ccgaaaagct gcgataaaac ccatacctgc ccgccgtgcc cggcgccgga
      841 actgctgggc ggcccgagcg tgtttctgtt tccgccgaaa ccgaaagata ccctgatgat
      901 tagccgcacc ccggaagtga cctgcgtggt ggtggatgtg agccatgaag atccggaagt
      961 gaaatttaac tggtatgtgg atggcgtgga agtgcataac gcgaaaacca aaccgcgcga
     1021 agaacagtat aacagcacct atcgcgtggt gagcgtgctg accgtgctgc atcaggattg
     1081 gctgaacggc aaagaatata aatgcaaagt gagcaacaaa gcgctgccgg cgccgattga
     1141 aaaaaccatt agcaaagcga aaggccagcc gcgcgaaccg caggtgtata ccctgccgcc
     1201 gagccgcgaa gaaatgacca aaaaccaggt gagcctgacc tgcctggtga aaggctttta
     1261 tccgagcgat attgcggtgg aatgggaaag caacggccag ccggaaaaca actataaaac
     1321 caccccgccg gtgctggata gcgatggcag cttttttctg tatagcaaac tgaccgtgga
     1381 taaaagccgc tggcagcagg gcaacgtgtt tagctgcagc gtgatgcatg aagcgctgca
     1441 taaccattat acccagaaaa gcctgagcct gagcccgggc aaaggcggcg gcggcagctg
     1501 gagccatccg cagtttgaaa aaggcggcgg cagcggcggc ggcagcggcg gcggcagctg
     1561 gagccatccg cagtttgaaa aataactcga gccttaggag atccggctgc taacaaagcc
     1621 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     1681 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca
     1741 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     1801 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     1861 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     1921 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     1981 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     2041 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
     2101 tcaaacatga gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     2161 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2221 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2281 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2341 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2401 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2461 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2521 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2581 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2641 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2701 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2761 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2821 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2881 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2941 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3001 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3061 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3121 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3181 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3241 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3301 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3361 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3421 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3481 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3541 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3601 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3661 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3721 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3781 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3841 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3901 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3961 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4021 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4081 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4141 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4201 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4261 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4321 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     4381 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     4441 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     4501 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc
     4561 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     4621 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     4681 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4741 agtgccaagc ttgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     4801 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     4861 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgc
//
