LOCUS T7_Trastuzumab_VH_TwinStrep_Plasmid 4915 bp ds-DNA circular 26-JUN-2024 DEFINITION . ACCESSION urn.local...h-hvqzy67 KEYWORDS . SOURCE ORGANISM . . COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers primer_bind 1..16 /created_by="User" /Sequence="CGGCCACGATGCGTCC" /label="T7LPfor PRIMER" promoter 51..69 /Imported_from="Pla sMapper" /Transferred_From="T7 prom" /Transferred_Similarity="100.00%" /label="T7 prom" 5'UTR 95..123 /created_by="User" /label="UTR1" motif 128..133 /created_by="User" /label="NdeI Site" motif 131..1498 /created_by="User" /modified_by="User" /label="Trastuzumab-VH-TS" motif 1499..1522 /created_by="User" /label="TwinStrepTag" motif 1523..1558 /created_by="User" /label="Linker" motif 1559..1582 /created_by="User" /label="TwinStrepTag" motif 1586..1591 /created_by="User" /label="XhoI Site" terminator 1639..1728 /created_by="User" /label="T7 Terminator" misc_feature complement(1814..1828) /created_by="User" /Sequence="CGACCGCTTTGGCCG" /label="T7LPrev PRIMER" /note="Geneious type: primer_bind_reverse" rep_origin 2509..3191 /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /modified_by="User" /label="ColE1 Origin" ORF complement(3289..4146) /created_by="User" /label="AmpR" promoter complement(4188..4216) /created_by="User" /label="Amp Promoter" ORIGIN 1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact 61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg 121 agatatacat atggaagtgc agctggtgga aagcggcggc ggcctggtgc agccgggcgg 181 cagcctgcgc ctgagctgcg cggcgagcgg ctttaacatt aaagatacct atattcattg 241 ggtgcgccag gcgccgggca aaggcctgga atgggtggcg cgcatttatc cgaccaacgg 301 ctatacccgc tatgcggata gcgtgaaagg ccgctttacc attagcgcgg ataccagcaa 361 aaacaccgcg tatctgcaga tgaacagcct gcgcgcggaa gataccgcgg tgtattattg 421 cagccgctgg ggcggcgatg gcttttatgc gatggattat tggggccagg gcaccctggt 481 gaccgtgagc agcgcgagca ccaaaggccc gagcgtgttt ccgctggcgc cgagcagcaa 541 aagcaccagc ggcggcaccg cggcgctggg ctgcctggtg aaagattatt ttccggaacc 601 ggtgaccgtg agctggaaca gcggcgcgct gaccagcggc gtgcatacct ttccggcggt 661 gctgcagagc agcggcctgt atagcctgag cagcgtggtg accgtgccga gcagcagcct 721 gggcacccag acctatattt gcaacgtgaa ccataaaccg agcaacacca aagtggataa 781 aaaagtggaa ccgaaaagct gcgataaaac ccatacctgc ccgccgtgcc cggcgccgga 841 actgctgggc ggcccgagcg tgtttctgtt tccgccgaaa ccgaaagata ccctgatgat 901 tagccgcacc ccggaagtga cctgcgtggt ggtggatgtg agccatgaag atccggaagt 961 gaaatttaac tggtatgtgg atggcgtgga agtgcataac gcgaaaacca aaccgcgcga 1021 agaacagtat aacagcacct atcgcgtggt gagcgtgctg accgtgctgc atcaggattg 1081 gctgaacggc aaagaatata aatgcaaagt gagcaacaaa gcgctgccgg cgccgattga 1141 aaaaaccatt agcaaagcga aaggccagcc gcgcgaaccg caggtgtata ccctgccgcc 1201 gagccgcgaa gaaatgacca aaaaccaggt gagcctgacc tgcctggtga aaggctttta 1261 tccgagcgat attgcggtgg aatgggaaag caacggccag ccggaaaaca actataaaac 1321 caccccgccg gtgctggata gcgatggcag cttttttctg tatagcaaac tgaccgtgga 1381 taaaagccgc tggcagcagg gcaacgtgtt tagctgcagc gtgatgcatg aagcgctgca 1441 taaccattat acccagaaaa gcctgagcct gagcccgggc aaaggcggcg gcggcagctg 1501 gagccatccg cagtttgaaa aaggcggcgg cagcggcggc ggcagcggcg gcggcagctg 1561 gagccatccg cagtttgaaa aataactcga gccttaggag atccggctgc taacaaagcc 1621 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg 1681 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca 1741 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag 1801 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa 1861 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg 1921 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc 1981 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg 2041 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg 2101 tcaaacatga gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 2161 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 2221 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 2281 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 2341 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2401 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2461 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2521 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2581 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2641 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2701 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 2761 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 2821 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 2881 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 2941 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 3001 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 3061 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 3121 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3181 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3241 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3301 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 3361 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 3421 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 3481 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 3541 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 3601 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 3661 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 3721 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 3781 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 3841 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 3901 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 3961 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 4021 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 4081 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 4141 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4201 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4261 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 4321 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 4381 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 4441 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 4501 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc 4561 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa 4621 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca 4681 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc 4741 agtgccaagc ttgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc 4801 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca 4861 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgc //