LOCUS       T7_Trastuzumab_VL_6XHis_Plasmid        4141 bp ds-DNA     circular     26-JUN-2024
DEFINITION  .
ACCESSION   urn.local...i-hvqzy67
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..70
                     /created_by="User"
                     /label="T7 Promoter"
     motif           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NdeI Site"
     ORF             131..808
                     /created_by="User"
                     /modified_by="User"
                     /label="VL-6XHis"
     motif           812..817
                     /created_by="User"
                     /label="XhoI"
     terminator      865..954
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(1040..1054)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      1735..2417
                     /created_by="User"
                     /label="ColE1 Origin"
     ORF             complement(2515..3372)
                     /created_by="User"
                     /label="AmpR"
     promoter        complement(3414..3442)
                     /created_by="User"
                     /label="Amp Promoter"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atggatattc agatgaccca gagcccgagc agcctgagcg cgagcgtggg
      181 cgatcgcgtg accattacct gccgcgcgag ccaggatgtg aacaccgcgg tggcgtggta
      241 tcagcagaaa ccgggcaaag cgccgaaact gctgatttat agcgcgagct ttctgtatag
      301 cggcgtgccg agccgcttta gcggcagccg cagcggcacc gattttaccc tgaccattag
      361 cagcctgcag ccggaagatt ttgcgaccta ttattgccag cagcattata ccaccccgcc
      421 gacctttggc cagggcacca aagtggaaat taaacgcacc gtggcggcgc cgagcgtgtt
      481 tatttttccg ccgagcgatg aacagctgaa aagcggcacc gcgagcgtgg tgtgcctgct
      541 gaacaacttt tatccgcgcg aagcgaaagt gcagtggaaa gtggataacg cgctgcagag
      601 cggcaacagc caggaaagcg tgaccgaaca ggatagcaaa gatagcacct atagcctgag
      661 cagcaccctg accctgagca aagcggatta tgaaaaacat aaagtgtatg cgtgcgaagt
      721 gacccatcag ggcctgagca gcccggtgac caaaagcttt aaccgcggcg aatgcggcgg
      781 cggcggcagc catcatcatc atcatcatta actcgagcct taggagatcc ggctgctaac
      841 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      901 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      961 tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     1021 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     1081 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     1141 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     1201 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     1261 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat
     1321 aagctgtcaa acatgagaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     1381 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1441 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1501 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1561 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2161 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2641 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2821 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3361 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3421 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3481 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3541 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3601 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3661 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     3721 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
     3781 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     3841 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3901 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3961 acggccagtg ccaagcttgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     4021 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     4081 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     4141 c
//
