LOCUS       T7_VHH_GFP4_6XHis__Linear_DNA         756 bp    DNA     linear   UNA 24-JUN-2024
DEFINITION  .
ACCESSION   urn.local...by-hvrl9sz
VERSION     urn.local...by-hvrl9sz
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /Sequence="CGGCCACGATGCGTCC"
                     /Hairpin_Tm="45.2"
                     /Tm="62.3"
                     /Self_Dimer_Tm="2.7"
                     /%GC="75.0"
                     /created_by="primer3"
                     /label="T7LPfor"
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /created_by="User"
                     /modified_by="User"
                     /label="T7 Promoter"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NcoI Site"
     misc_feature    130..510
                     /locus_tag="VHH_GFP4"
                     /ApEinfo_label="VHH_GFP4"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="VHH_GFP4"
     motif           514..519
                     /created_by="User"
                     /label="XhoI Site"
     terminator      567..656
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(742..756)
                     /Sequence="CGACCGCTTTGGCCG"
                     /Hairpin_Tm="44.6"
                     /Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /%GC="73.3"
                     /created_by="primer3"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tggatcaggt gcagctggtg gaaagcggcg gcgcgctggt gcagccgggc
      181 ggcagcctgc gcctgagctg cgcggcgagc ggctttccgg tgaaccgcta tagcatgcgc
      241 tggtatcgcc aggcgccggg caaagaacgc gaatgggtgg cgggcatgag cagcgcgggc
      301 gatcgcagca gctatgaaga tagcgtgaaa ggccgcttta ccattagccg cgatgatgcg
      361 cgcaacaccg tgtatctgca gatgaacagc ctgaaaccgg aagataccgc ggtgtattat
      421 tgcaacgtga acgtgggctt tgaatattgg ggccagggca cccaggtgac cgtgagcagc
      481 ggcagcggca gccatcatca tcatcatcat taactcgagc cttaggagat ccggctgcta
      541 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      601 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      661 gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
      721 gaagcgagca ggactgggcg gcggccaaag cggtcg
//
