LOCUS       T7_VHH_GFP4_6XHis_Plasmid        3843 bp ds-DNA     circular     26-JUN-2024
DEFINITION  .
ACCESSION   urn.local...8o-hvrd656
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     promoter        51..69
                     /created_by="User"
                     /modified_by="User"
                     /label="T7 Promoter"
     5'UTR           95..123
                     /created_by="User"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NcoI Site"
     misc_feature    130..510
                     /locus_tag="VHH_GFP4"
                     /ApEinfo_label="VHH_GFP4"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="VHH_GFP4"
     motif           514..519
                     /created_by="User"
                     /label="XhoI Site"
     terminator      567..656
                     /created_by="User"
                     /label="T7 Terminator"
     misc_feature    complement(742..756)
                     /Sequence="CGACCGCTTTGGCCG"
                     /Hairpin_Tm="44.6"
                     /Tm="59.5"
                     /Self_Dimer_Tm="None"
                     /%GC="73.3"
                     /created_by="primer3"
                     /label="T7LPrev"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(742..756)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      1437..2119
                     /created_by="User"
                     /label="ColE1 Origin"
     ORF             complement(2214..3074)
                     /created_by="User"
                     /label="AmpR"
     promoter        complement(3116..3144)
                     /created_by="User"
                     /label="Amp Promoter"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tggatcaggt gcagctggtg gaaagcggcg gcgcgctggt gcagccgggc
      181 ggcagcctgc gcctgagctg cgcggcgagc ggctttccgg tgaaccgcta tagcatgcgc
      241 tggtatcgcc aggcgccggg caaagaacgc gaatgggtgg cgggcatgag cagcgcgggc
      301 gatcgcagca gctatgaaga tagcgtgaaa ggccgcttta ccattagccg cgatgatgcg
      361 cgcaacaccg tgtatctgca gatgaacagc ctgaaaccgg aagataccgc ggtgtattat
      421 tgcaacgtga acgtgggctt tgaatattgg ggccagggca cccaggtgac cgtgagcagc
      481 ggcagcggca gccatcatca tcatcatcat taactcgagc cttaggagat ccggctgcta
      541 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      601 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      661 gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
      721 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg
      781 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg
      841 tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
      901 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
      961 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
     1021 ataagctgtc aaacatgaga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     1081 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1141 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1201 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2161 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2221 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2281 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2341 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2401 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2461 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2521 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2581 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2641 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2701 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2761 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2821 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2881 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2941 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3001 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3061 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3181 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3241 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     3301 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3361 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     3421 tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc
     3481 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     3541 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     3601 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     3661 cgacggccag tgccaagctt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     3721 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     3781 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     3841 tgc
//
