LOCUS       T7_deGFP_Control_Linear_DNA        1050 bp    DNA     linear   UNA 24-JUN-2024
DEFINITION  .
ACCESSION   urn.local...ak-hvrkfz1
VERSION     urn.local...ak-hvrkfz1
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     misc_feature    51..70
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="T7 Promoter"
     misc_feature    95..123
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NcoI Site"
     misc_feature    130..804
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="deGFP"
     motif           808..813
                     /created_by="User"
                     /label="XhoI Site"
     misc_feature    861..950
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="T7 terminator"
     misc_feature    complement(1036..1050)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tggagctttt cactggcgtt gttcccatcc tggtcgagct ggacggcgac
      181 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      301 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      541 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      601 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      721 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      781 gagttcgtga ccgccgccgg gatctaactc gagccttagg agatccggct gctaacaaag
      841 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      901 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc
      961 cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg
     1021 agcaggactg ggcggcggcc aaagcggtcg
//
