LOCUS       T7_deGFP_Control_Plasmid        4137 bp ds-DNA     circular     26-JUN-2024
DEFINITION  .
ACCESSION   urn.local...13-hvr1rbx
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:0.
FEATURES             Location/Qualifiers
     primer_bind     1..16
                     /created_by="User"
                     /Sequence="CGGCCACGATGCGTCC"
                     /label="T7LPfor PRIMER"
     misc_feature    51..70
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="T7 Promoter"
     misc_feature    95..123
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="UTR1"
     motif           128..133
                     /created_by="User"
                     /label="NcoI Site"
     misc_feature    130..804
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="deGFP"
     motif           808..813
                     /created_by="User"
                     /label="XhoI Site"
     misc_feature    861..950
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="T7 terminator"
     misc_feature    complement(1036..1050)
                     /created_by="User"
                     /Sequence="CGACCGCTTTGGCCG"
                     /label="T7LPrev PRIMER"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      1731..2413
                     /created_by="User"
                     /label="ColE1 Origin"
     CDS             complement(2511..3170)
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="AmpR"
     promoter        complement(3410..3438)
                     /created_by="User"
                     /label="Amp Promoter"
ORIGIN      
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tggagctttt cactggcgtt gttcccatcc tggtcgagct ggacggcgac
      181 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      301 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      541 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      601 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      721 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      781 gagttcgtga ccgccgccgg gatctaactc gagccttagg agatccggct gctaacaaag
      841 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      901 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc
      961 cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg
     1021 agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga
     1081 aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa
     1141 tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
     1201 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
     1261 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc
     1321 tgtcaaacat gagaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     1381 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1441 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1501 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1561 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1621 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1681 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1741 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1801 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1861 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1921 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1981 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2041 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2101 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2161 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2221 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2281 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2341 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2401 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2461 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2521 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2581 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2641 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2701 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2761 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2821 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2881 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2941 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3001 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3061 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3121 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3181 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3241 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3301 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3361 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3421 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3481 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3541 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     3601 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     3661 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     3721 gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata ccgcacagat
     3781 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     3841 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3901 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3961 ccagtgccaa gcttgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     4021 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     4081 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgc
//
