A new hybridization capture kit has been developed to enhance genomic sequencing for the Annonaceae plant family, targeting 799 low-copy genes. This kit combines 469 genes from the original Annonaceae probe with 334 genes from the universal Angiosperms353 kit. The study compares results from the original Angiosperms353 kit with the custom approach using various specimens. Findings show that the Annonaceae799 kit achieves high recovery rates, and while off-target reads were noted, the genes from the Angiosperms353 panel significantly outperformed those from the original Annonaceae kit in terms of size, on- and off-target regions, and the number of parsimony-informative sites. This integration not only improves the utility of the probe kit for species-level phylogenomics and within-species analysis but also broadens access to new genes for future phylogenetic and population studies.

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