Overview
Just add DNA to express analysis-ready antibodies or target proteins in hours!
In cell-free protein expression systems, gene transcription (TX) and translation (TL) are unbound from constraints required for cell culture-based processes. myTXTL systems offer a versatile and easy-to-use cell-free expression platform for protein synthesis, enzyme engineering, and synthetic biology processes in industry and academia.
Our cell-free myTXTL systems allow rapid and parallel production of soluble or toxic proteins and other complex biological structures, like bacteriophages, under open-reaction conditions. This enables easy manipulation, addition of chaperone molecules and straightforward downstream processing of synthesized proteins, enzymes and phages.
- Accelerate your workflow with cell-free protein expression tools that let you screen more variants in less time. Accomplish in two days what would take 3 weeks with in-vivo methods.
- Scale up production with high-throughput solutions that enable increased yield with reliable performance.
- Achieve superior results with tools designed for ease of use, backed by scientific experts available to help optimize your protocols and ensure your success.
- Perform protein expression not possible with in-vivo methods, such as synthesizing toxic proteins that prevent cell survival.
Technology
myTXTL utilizes the endogenous transcription (TX) and translation (TL) machinery from E. coli. Protein expression is initiated by simply adding DNA to the master mix and target protein activity can be measured in as little as 15 minutes.
Based on a system originally developed by Prof. Vincent Noireaux at the University of Minnesota, our revolutionary myTXTL technology has been further refined for greater robustness and manufacturability to deliver reliable, consistent performance.
Two systems, choose what’s right for you:
- myTXTL Pro Kit: High yield system for proteins that do not require disulfide bond formation
- myTXTL Antibody/DS Kit: Engineered for the expression of antibodies and disulfide-bond containing proteins, can also express proteins without disulfide bonds
Looking for a different myTXTL product? Previous items will be retired in August 2024. Learn more here.
Advantages
- Use linear or plasmid DNA templates – skip cloning!
- Supports any promoter system used for protein expression in E. coli, including T7, no need for recloning into a specialized plasmid.
- High throughput applications, 10s or 1000s of reactions.
- Open reaction allows dilution into activity assays, no purification.
- Adjustable reaction volumes to suit your needs.
Applications
- Antibody discovery/screening
- CRISPR-Cas and transposase variant screening and activity assays
- Synthetic biology: gene circuits, rebooting for bacteriophage, metabolic pathway optimization
- Protein engineering
- Rapid data generation for informing AI/ML models
Resources
Product Literature
Poster
myTXTL Cell-Free Expression of Antibodies and Antibody Fragments – PEGS 2024
Need assistance?
Our scientists are ready to help you select the right Daicel Arbor Biosciences product for your research needs.