Two critical bottlenecks exist in high-throughput protein engineering and expression workflows: DNA synthesis and protein expression/analysis. Plasmid DNA synthesis can take 2-3 weeks, followed by 2-3 additional days to express and purify protein for analysis.
myTXTL® cell-free protein expression solutions from Daicel Arbor Biosciences help relieve these bottlenecks by:
- Expressing protein immediately upon adding DNA template, yielding enough protein for analysis in hours not days.
- Supporting linear DNA templates to reduce time and costs compared to cloning DNA into plasmids (plasmid DNA is also supported).
- Eliminating the need for additional protein purification because the open reaction environment offers immediate access to the protein for activity assays.
Our myTXTL cell-free products work with existing expression vectors used in E. coli and scale from 24 to thousands of reactions.