It may be necessary at times to add components to the myTXTL system from sources that contain material of unknown effect in the myTXTL system, the following is a guide for what chemicals/reagents may be tolerated without a loss in performance:
1) Glycerol is tolerated up to 0.1% of myTXTL reaction volume
2) DMSO is tolerated up to 1% of myTXTL reaction volume
3) EDTA is tolerated up to 0.1 mM of myTXTL reaction volume
4) Tris-HCl (pH 8) is tolerated up to 50 mM of myTXTL reaction volume
5) CaCl2 is tolerated up to 1 mM of myTXTL reaction volume
6) MgCl2 is tolerated up to 1 mM of myTXTL reaction volume
7) NaCl is tolerated up to 50 mM of myTXTL reaction volume