Name: myBaits Expert Wheat Exome
Brand: Arbor Biosciences

myBaits Expert — Predesigned Panels

myBaits Expert Wheat Exome

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Enrich for over 250 megabases of high-confidence exons from a wide variety of wheat cultivars.

The myBaits^® Expert Wheat Exome hybridization capture panel was developed in collaboration with the International Wheat Genome Sequencing Consortium (IWGSC) using the most up to date genome assembly and annotation set of Chinese Spring. The panel enriches for the complete high-confidence exon-annotated genome in hexaploid wheat, retrieving over 250 megabases of CDS and CDS-proximate regions of the greater than 15 GB Triticum aestivum genome.

Developed with the International Wheat Genome Sequencing Consortium
2 million probes targeting >200 megabases of high-confidence exons
Compatible with hexaploid and tetraploid cultivars of wheat
Compatible with short- and long-read sequencing

This panel saves the exome-focused researcher orders of magnitude in sequencing and analysis costs compared to whole genome sequencing. Available to order as a standalone myBaits enrichment kit, or if a complete solution is desired, the myReads^® NGS services team at Daicel Arbor Biosciences is available to perform library preparation, target capture, and sequencing for your entire project. Included bioinformatics services are powered by our partnership with Curio Genomics to deliver an end-to-end solution for wheat exome sequencing. Contact us today to get started on a comprehensive wheat sequencing project that aligns with your budget and project goals.

Ordering Information

Size

Cat#

Qty

Price

myBaits Expert Wheat Exome v1 Kit + myCurio Reports

309108.v5

$4,390

Add to Cart

myReads Wheat Exome Sequencing + myCurio Reports, 1-95 samples

719095

$550

Add to Cart

myReads Wheat Exome Sequencing + myCurio Reports, 96-383 samples

719383

$500

Add to Cart

myReads Wheat Exome Sequencing + myCurio Reports, 384+ samples

719384

$475

Add to Cart

The myBaits Expert Wheat Exome kit is available in-stock for immediate shipping.

Curio Genomics
Overview
Performance
Resources

Curio Genomics

Bioinformatics Services & Analysis

We have partnered with Curio Genomics on bioinformatics analysis of the myBaits Expert Wheat Exome panel in order to provide a complete solution for wheat exome sequencing. In collaboration with the IWGSC, we have added the RefSeq v1.0 wheat genome assembly to Curio’s platform in order to deliver a streamlined solution for analyzing sequencing data.

Sequencing data processed by myReads NGS Services team
Available as a full service sequencing project or paired with the myBaits kit
Compatible with hexaploid and tetraploid cultivars of wheat
Easily review and share analysis within Curio platform

The platformstreamlines bioinformatics analysis by moving beyond the typical pipeline approach of serial processes through parallel processing sequencing data. Curio’s unique incremental and parallel approach to processing wheat exome data allows users to compare between samples, change analytical settings, and apply different filters all in real time, with the results immediately at their fingertips. Bioinformatics services are available from Daicel Arbor Biosciences through the myReads NGS Services as a full service project, from DNA to variant calling, or for samples processed in your own lab with the myBaits Expert Wheat Exome Panel.

Curio Genomics Platform

Curio Platform Wheat Variants Fig. 2. The Curio Genomics platform provides real time editing and analysis of wheat exome panel data. This intuitive, user-friendly interface provides rapid processing and review of NGS data, and is available for wheat exome data analysis either bundled with a full-service package, or as a standalone service.

Overview

Sequence the Exome of any Wheat Cultivar

NGS read alignments to 4 wheat cultivars for the gamma gliadin member 1 gene, showing exon and probe locations.

Fig. 1. The Wheat Exome panel provides reliable capture of diverse sequences. myBaits chemistry allows highly reproducible coverage across divergent cultivars. Unique read coverage and polymorphisms in 4 distinct cultivars are shown for the gamma gliadin member 1 gene.

Fig. 2. The Wheat Exome panel can be used on both hexaploid and tetraploid cultivars. Although the panel is designed for all high-confidence exons from the hexaploid Chinese Spring IWGSCv1 reference genome, it has high efficiency for other types of wheat as well. This figure depicts both the predicted vs. observed capturable portions of exomes from hexaploid and tetraploid wheat cultivars. Download Arbor Bio’s poster from the 2020 PAG XXVIII meeting to review performance data of the Wheat Exome kit in tetraploid wheat samples.

Performance

Reliable and Comprehensive Sequencing Coverage

Fig 3. Target coverage (left, green) and reads on-target (right, blue) for 8 cultivar samples captured in a single reaction. With only ~18Gbp sequencing effort per sample, the Wheat Exome v1 kit consistently provides an average 34X unique read coverage across >200 Mbp of target space, overlapping >95% of the IWGSC v1.1 high-confidence exon annotations.

Table 1. Summary design and read coverage metrics for the Wheat Exome v1 kit, demonstrating consistently high average read coverage across >250 Mb of total genomic target space, including 97% of the high-confidence exon annotations in the IWGSCv1 wheat genome assembly, for the target+200bp padded target interval set.

Resources