The myTXTL® T7 Expression kit is based on our LS70 Master Mix technology utilized in the myTXTL Linear DNA Expression Kit, and has been engineered for maximum robustness and reliable performance when using T7 promoter-driven constructs. This all-in-one solution contains a P70a-T7rnap HP plasmid which delivers continuous production of T7 RNA polymerase in addition to an E. coli-based master mix containing the cellular machinery, energy buffer, and amino acids premixed for in vitro protein production in a single tube. Simply start protein synthesis by adding a DNA template containing a T7 promoter. A positive control plasmid with one of our highest expressing green fluorescent proteins (GFP) is included in the kit for easy confirmation of proper protein synthesis conditions.
As in vitro protein synthesis in the myTXTL cell-free system relies on the endogenous primary sigma factor 70 (σ70) of E. coli, a σ70-specific promoter is used to drive expression of T7 RNA polymerase in the myTXTL T7 Expression kit. This co-expression of the P70a-T7rnap HP plasmid delivers continuous expression of T7 RNA polymerase for robust gene expression from traditional T7 expression system constructs.
- All-in-one Solution – Simply mixing template DNA and ready-to-use myTXTL Master Mix.
- High-throughput screening – Process more samples within a single experiment.
- Various inputs – Start from linear or circular DNA templates.
- Highly Controllable – Easily adjust experimental parameters.
