Ultraconserved Elements (UCEs) are highly conserved genomic regions which are flanked by more divergent sequences.
These key areas have been identified and exploited as ideal target capture candidates for phylogenetic research on novel and non-model species, as many genomic regions are shared across large taxonomic ranges. Sets of UCE loci have been identified for various taxonomic groups, including vertebrates, insects, and many more. Arbor Biosciences offers a number of popular UCE panels as readily stocked kits available for immediate shipment at lower per-reaction costs. Additional info on the utility of UCEs for genetic research can be found at UltraConserved.org.
Predesigned – Designed by experts and ready for immediate use
Proven results – Ever expanding list of peer-reviewed publications
Analysis pipelines – Simple, user-friendly data analysis
Cost-effective – Use one bait set across multiple distantly-related taxa
myBaits UCE Acanthomorph 1Kv1
Kit for enrichment of 1,314 UCE loci present in Acanthomorph (Percomorph) fishes.
Figure 1. Example alignment of a 120bp UCE locus from the Tetrapods 5K baitset compared to the region containing the top BLAST hit in genomes of chicken (Gallus gallus), human, mouse (Mus musculus), and frog (Xenopus laevis. Individual bases are colored by degree of similarity between sequences (dark = high, light = low). The central 120bp UCE region is strongly conserved across all taxa, whereas immediate flanking regions are more divergent.
We also recommend reviewing the published UCE literature associated with your panel of choice to see how other researchers utilized their myBaits reagents, including factors such as library insert length, sequencing depth, hyb/wash temp, and more. To download past myBaits manual versions, visit the myBaits Manual archive page.
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