Ultraconserved Elements (UCEs) are highly conserved genomic regions which are flanked by more divergent sequences.
myBaits Expert UCE
These key areas have been identified and exploited as ideal target capture candidates for phylogenetic research on novel and non-model species, as many genomic regions are shared across large taxonomic ranges. Sets of UCE loci have been identified for various taxonomic groups, including vertebrates, insects, and many more. Daicel Arbor Biosciences offers a number of popular UCE panels as readily stocked kits available for immediate shipment at lower per-reaction costs. Additional info on the utility of UCEs for genetic research can be found at UltraConserved.org.
Predesigned – Designed by experts and ready for immediate use
Proven results – Ever expanding list of peer-reviewed publications
Analysis pipelines – Simple, user-friendly data analysis
Cost-effective – Use one bait set across multiple distantly-related taxa
Ordering Information
Size
Cat#
Qty
Price
myBaits UCE Acanthomorph 1Kv1
Kit for enrichment of 1,314 UCE loci present in Acanthomorph (Percomorph) fishes.
Kit for enrichment of 2,524 UCE loci present in Hymenoptera and 16 nuclear exons (12 genes) commonly sequenced in insects. Subset of the ?principal? set, including baits from ant genomes only.
Kit for enrichment of 2,545 UCEs present in Hymenoptera and 12 nuclear exons (7 genes) commonly sequenced in insects. Subset of the Principal set, including baits from ant and bee genomes only.
This set of 6,737 baits (120 mer) targets 2,708 UCE loci present in Ostariophysan fishes. The baits can be downloaded from Figshare. The baitset was first described in: Faircloth BC, Alda F, Hoekzema K, Burns MD, Oliveira C, Albert JS, Melo BF, Ochoa LE, Roxo FF, Chakrabarty P, Sidlauskas BL, Alfaro ME (2018). A target enrichment bait set for studying relationships among ostariophysan fishes. BioRxiv.
UCEs: Ray-finned Fish (Actinopterygians) 0.5K version 1
UCEs: Insect Hymenoptera 2.5K version 2, Ant-Bee-Specific
This set of 9,332 baits (120 mer) targets 2,545 UCE loci present in Hymenoptera and 12 nuclear exons (7 genes) commonly sequenced in insects. The UCE baits are a subset of the more diverse “principal” bait set, including baits from ant and bee genomes only. The baitset can be downloaded here. The baitset was first described in: Grab, H. et al. (2019) Agriculturally dominated landscapes reduce bee phylogenetic diversity and pollination services. Science 363(6424): 282-284.
Figure 1. Example alignment of a 120bp UCE locus from the Tetrapods 5K baitset compared to the region containing the top BLAST hit in genomes of chicken (Gallus gallus), human, mouse (Mus musculus), and frog (Xenopus laevis. Individual bases are colored by degree of similarity between sequences (dark = high, light = low). The central 120bp UCE region is strongly conserved across all taxa, whereas immediate flanking regions are more divergent.
Resources
myBaits Kit Protocol
Current Version: v5
myBaits Manual v5 For kits shipped starting in Sept 2020, with Cat#’s ending in “.v5”
We also recommend reviewing the published UCE literature associated with your panel of choice to see how other researchers utilized their myBaits reagents, including factors such as library insert length, sequencing depth, hyb/wash temp, and more. To download past myBaits manual versions, visit the myBaits Manual archive page.
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