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Home » Products » Cell-Free Protein Expression

Cell-free Protein Expression

Accelerating research for antibody discovery and protein engineering

Daicel Arbor Biosciences provides innovative tools that dramatically accelerate protein discovery and development, overcoming the limitations of traditional cell-based processes. Our myTXTL® protein expression products enable simpler workflows and faster results, accelerating discovery to get you the data you want as soon as possible.

myTXTL® Pro Kit

myTXTL® Antibody/DS Kit

Overview

Just add DNA to express analysis-ready antibodies or target proteins in hours!

In cell-free protein expression systems, gene transcription (TX) and translation (TL) are unbound from constraints required for cell culture-based processes. myTXTL systems offer a versatile and easy-to-use cell-free expression platform for protein synthesis, enzyme engineering, and synthetic biology processes in industry and academia.

Our cell-free myTXTL systems allow rapid and parallel production of soluble or toxic proteins and other complex biological structures, like bacteriophages, under open-reaction conditions. This enables easy manipulation, addition of chaperone molecules and straightforward downstream processing of synthesized proteins, enzymes and phages.

  • Accelerate your workflow with cell-free protein expression tools that let you screen more variants in less time. Accomplish in two days what would take 3 weeks with in-vivo methods.
  • Scale up production with high-throughput solutions that enable increased yield with reliable performance.
  • Achieve superior results with tools designed for ease of use, backed by scientific experts available to help optimize your protocols and ensure your success.
  • Perform protein expression not possible with in-vivo methods, such as synthesizing toxic proteins that prevent cell survival.

Technology

myTXTL utilizes the endogenous transcription (TX) and translation (TL) machinery from E. coli. Protein expression is initiated by simply adding DNA to the master mix and target protein activity can be measured in as little as 15 minutes.

Based on a system originally developed by Prof. Vincent Noireaux at the University of Minnesota, our revolutionary myTXTL technology has been further refined for greater robustness and manufacturability to deliver reliable, consistent performance.

Two systems, choose what’s right for you:

  • myTXTL Pro Kit: High yield system for proteins that do not require disulfide bond formation
  • myTXTL Antibody/DS Kit: Engineered for the expression of antibodies and disulfide-bond containing proteins, can also express proteins without disulfide bonds

Looking for a different myTXTL product? Previous items were retired in August 2024. Learn more here.

Advantages

  • Use linear or plasmid DNA templates – skip cloning!
  • Supports any promoter system used for protein expression in E. coli, including T7, no need for recloning into a specialized plasmid.
  • High throughput applications, 10s or 1000s of reactions.
  • Open reaction allows dilution into activity assays, no purification.
  • Adjustable reaction volumes to suit your needs.

Applications

  • Antibody discovery/screening
  • CRISPR-Cas and transposase variant screening and activity assays
  • Synthetic biology: gene circuits, rebooting for bacteriophage, metabolic pathway optimization
  • Protein engineering
  • Rapid data generation for informing AI/ML models

Resources

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Product Literature

Product Sheet

myTXTL Antibody/DS Kit for Cell-Free Protein Expression

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Product Literature

Product Sheet

myTXTL Pro Kit for Cell-Free Protein Expression

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Product Literature

Poster

myTXTL Cell-Free Expression of Antibodies and Antibody Fragments – PEGS 2024

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Video

Webinar

myTXTL®: Breaking Out of the Cell to Enable High-Throughput Protein Engineering

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Video

Webinar

Next Generation AI Driven Protein Engineering Workflows – with Strateos

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Video

Webinar

Accelerating High-Throughput Protein Engineering Workflows – with IDT

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Application Note

Arzeda’s next-generation protein design workflow powered by the myTXTL cell-free protein expression system enables smarter and faster discovery – with Arzeda

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Application Note

An accelerated protein engineering workflow utilizing linear DNA in a cell-free expression system – with IDT

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Video

Education

Introduction to myTXTL cell-free protein expression

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Application Note

Miniaturization and rapid processing of TXTL reactions using acoustic liquid handling – with Beckman Coulter

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Product Literature

Poster

Miniaturization and rapid processing of TXTL reactions using acoustic liquid handling – with Labcyte

OverviewTechnologyAdvantagesApplicationsResources

Need assistance?

Our scientists are ready to help you select the right Daicel Arbor Biosciences product for your research needs.

Contact Us

Daicel Arbor Biosciences
5840 Interface Dr. Suite 101,
Ann Arbor, MI 48103
1.734.998.0751
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  • Products
    • Targeted NGS
      • myBaits Custom Hybridization Capture Kits
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      • Library Preparation Kits
    • Cytogenetics ISH
      • myTags Custom Probes
      • myTags Predesigned Probes
    • Cell-Free Protein Expression
      • myTXTL Pro Kit
      • myTXTL Antibody/DS Kit
  • Lab Services
    • Sequencing Services
      • DNA & RNA Extraction
      • Library Preparation
      • Targeted Sequencing
      • NGS Platform Support
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      • Forensics & ancient DNA
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      • Long-read sequencing
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