myTags – In-Situ Hybridization Probes
myTags Custom – Immortal and Labeled ISH Probes
Light up your cytogenomics research with flexible myTags® Custom probes for any in-situ hybridization application.

Custom FISH Probes – myTags

Daicel Arbor Biosciences announces our all new myTags Custom lineup, featuring our unique Indexed Synthesis and Labeling Service options that perfectly complement our high-quality oligo probe synthesis capabilities.

The myTags Custom portfolio provides maximum flexibility for all your in-situ hybridization needs, no matter your application or project scale.

  • Immortal amplifiable or labeled ready-to-use formats
  • Custom probe design service included
  • Project design support with expertise in complex genomes and challenging substrates
  • NEW Indexed Flex Synthesis is more cost effective for ordering multiple probe sets of varying sizes, perfect for multiplex (F)ISH studies
  • NEW Labeling Service offers highest value for multiple labeling options

No other manufacturer matches the expertise, flexibility, and multiplex options of the myTags Custom product family to meet all your oligo FISH needs. Connect with us today to get started on your next in-situ hybridization project.

Applications

Fluorescent In Situ Hybridization (FISH)
DNA-FISH
RNA-FISH
Cryo-FISH
Flow cytometry
Fluorescent reporters
Microarray signal enhancement



Overview

(F)ISH Probes for Any Application

myTags Custom probes from Daicel Arbor Biosciences are THE go-to source for ready-to-use, highly specific fluorescent probes for your next in-situ hybridization (ISH) project. myTags probes have been successfully used in specialized applications including 3D DNA-FISH, Cryo-FISH, and RNA-FISH in a variety of cell types. Our bioinformaticians armed with our proprietary design tools can always accommodate your self- or pre-designed probe sets into our system.

Whether you wish to label your own probes or have us do it for you, our experts will craft a solution to match your workflow and experimental needs.

Request a Quote Today

Contact Our Experts
FAQs
What is the difference between Single Synthesis and Indexed Synthesis?

For standalone orders and/or complex probe designs requiring up to 100K+ oligos, our Single Synthesis option provides maximum value. For smaller and/or multiple designs, our new Indexed Synthesis option maximizes both flexibility and cost-effectiveness. All probesets are delivered individually (200 ng minimum yield) and include composition verification via next-generation sequencing to confirm the quality of probe synthesis.

Arbor’s experts will assist you with determining which of our product configurations is most appropriate for your experimental goals and target type(s). You will have full transparency and control over the entire process. Contact us today to get started with your next project and experience the many benefits of using synthetic custom (F)ISH probes.

With Arbor’s flexible oligo synthesis manufacturing technology, we offer two configurations:

Single Synthesis

  • Oligo pools containing 1 probeset design
  • Can contain up to 108K unique probe sequences per design (larger pools may be available)
  • 6 different sizes, depending on total number of probes in design: 1-1.8K, 1.8K-4K, 4K-27K, 27K-54K, 54K-81K, or 81K-108K probe sequences

Indexed Synthesis

  • Oligo pools containing 2-10 probeset designs per synthesis event (up to 27K total oligos)
  • If >10 designs and/or >27K oligos are needed, add additional oligo pools to scale up
  • Oligos are separated into individual probesets via PCR deconvolution

Short index sequences are present on the final probe oligos, but are short and not expected to interfere with downstream (F)ISH protocols.

For all myTags Custom orders, final oligo pool composition is verified by next-generation sequencing (NGS), and oligos are delivered as individual probesets.

Add on myTags Labeling Services to get your (F)ISH experiments started quickly!

How does Indexed Synthesis work?

Steps of myTags Custom production using the Indexed Synthesis technique:

  1. Probes are designed for each target region separately
  2. All probe sequences (up to 27K total) are synthesized together
  3. Using unique indexes, each individual probeset is separated into individual oligo pools via PCR deconvolution
  4. Composition of oligo pools is verified with next-generation sequencing (NGS)

Short index sequences are present on the final probe oligos, but are short and not expected to interfere with downstream (F)ISH protocols.

Do you provide design assistance for myTags Custom probes?

We offer complementary (F)ISH probe bioinformatic design service using our proprietary design algorithms for most types of (F)ISH projects. Please contact us with a brief description of your project, including the name of your study species, genomic coordinates, and any additional information.
What probe design recommendations do you make for successful FISH assays?

Generally we recommend probe densities between 3-10 probes per kilobase for target regions larger than 50kb. For target regions between 10-50kb, probe densities should be on the higher end of that range, and we may recommend using multiple fluorophores per probe to boost the signal.
Can you design probes for unassembled genomes?

We can work with any sequence to design probes. Please contact us with a brief description of your project, including the name of your study species, genomic coordinates, and any additional information.
Can you manufacture Oligopaints probe libraries?

Yes, we can synthesize immortal probe libraries that can be labeled using the Oligopaints labeling method. Please note these probe libraries are not compatible with the standard myTags labeling protocol due to sequence requirements of the Oligopaints method.
How many FISH assays can you get from 700pmol of labeled probes?

The number of assays per library depends on a number of factors including the probe density of your library, the size of your target region, the number of probes in the library, and the FISH protocol. Generally we recommend starting with 10pmol of labeled probes per standard FISH slide and then modifying the input amount based on the initial results.
Which dyes do you recommend?

Atto-550 is our most popular dye labeling option, followed by Atto-488, Atto-594, and Atto-647N. Biotin, Digoxigenin and 6FAM are also popular options that work well in multi-color FISH assays.
Do you offer other labeling options than what is listed on the website?

We may be able to accommodate other labeling options, please contact us for availability.
What protocol should I use with my myTags Custom probes?

The latest recommended protocols for both labeled and immortal probe libraries can be found in the Resources tab, but in general, myTags FISH libraries are compatible with most FISH protocols. Please contact us for specific recommendations.
How quickly can you ship my order?

We generally ask for up to 3-4 weeks after an order is placed to ship myTags libraries.
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