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myTags Applications and Image Gallery

Below are just a few categories using various applications for myTags. Hover over each image to see a description.

The publications for the images may be viewed here.

3D Visualization and Analysis of Genome Organization

Coupling fluorescence in situ hybridization (FISH) with 3D microscopy and image reconstruction to study spatial organization of the nuclear genome, chromatin topology and interactions between chromosomes/chromatins, which play important roles in transcriptional and epigenetic regulation of the genome function.

Chromosome Structure and Arrangement

Using FISH-based assay to reveal chromosome structures and rearrangements including translocations, segmental duplications/deletions, and presence-absence variations, which is essential for studying recombination between chromosomes, chromosome synteny and evolution, massive gene duplication and amplification events, etc.

Chromosome Identification, Karyotyping and Chromosome Painting

Using oligo-FISH to develop chromosome identification/barcode system, allowing karyotyping based on individually identified chromosomes and studies of chromosome-scale genetic adaptation and evolution; Chromosome/haplotype-specific painting using chromosome or haplotype-specific oligo probes can be applied to research in chromosomal evolution, monitoring chromosome pairing and transmission and assessing genomic sequence assembly.

Oligonucleotides Based Fluorescent in situ Hybridization (Oligo-FISH)

Oligo-FISH, which utilizes custom-designed synthetic single stranded oligonucleotides probes, is the fundamental technique required for chromosome identification, chromosome painting, chromosome rearrangement and study of three-dimensional chromatin organization, etc.

Evaluation of Gene Expression and Regulation

Visualization of chromatin interactions and spatial arrangement/localization of target genes or mRNA transcripts using oligo-FISH allows for investigation of transcriptional and epigenetic regulation activities.

Super-resolution Imaging of Nuclear Organizations and Structures

Oligo-FISH in combination with super-resolution imaging techniques allows for analyzing the subcellular localization and spatial arrangement of targeted DNA sequences and RNA transcripts at the single cell level.

To view publications represented by images, click here.

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