myBaits Expert — Predesigned Panels
myBaits Expert – Plant – Angiosperms-353
This new panel enriches 100's of single-copy genes orthologous across all angiosperms (flowering plants).

Combining the flexible hybridization power of in-solution target capture with an expertly selected set of orthologous locus sequences, this new probe set has been demonstrated to enrich hundreds of putatively single-copy protein-coding genes across a broad range of angiosperms (flowering plants). Probes were designed from 353 loci, each with 5-15 representative sequences from across all angiosperms which were selected using a novel “k-medoids clustering approach” to maximize taxonomic breadth of the design (Johnson, Pokorny, Dodsworth et al 2018, Systematic Biology).

These probes are broadly applicable for phylogenetic research across all flowering plants, and the kit is available from Arbor Biosciences as an in-stock catalog kit available for immediate shipment at a low per-reaction cost. As with all myBaits kits, the Angiosperms353 panel is provided as a complete solution target capture kit, including buffers, blockers, and baits, along with an easy-to-use protocol. Or if you would prefer to outsource the work, our myReads NGS service expert scientists are available to perform library preparation, target capture, and sequencing for your entire project.

  • Predesigned Panel – Designed by expert plant geneticists
  • Proven results – Demonstrated utility across broad taxonomic range
  • Freely accessible pipelines – Simple, user-friendly data analysis
  • Cost-effective – Use one bait set across multiple distantly-related taxa
Ordering Information
Size
Cat#
Qty
Price
myBaits Expert Angiosperms 353 v1
Kit for enrichment of >350 putatively single-copy nuclear loci from flowering plants (Angiosperms). For phylogenetics.
308108
$1,000
Available in-stock for immediate shipment.


Overview

Enrich Hundreds of Angiosperm Exons and their Flanking Regions

For more information about the design and performance of this probeset, please review the following primary sources:

The Publication

M Johnson, L Pokorny, S Dodsworth, LR Botigue, RS Cowan, A Devault, WL Eiserhardt, N Epitawalage, F Forest, JT Kim, JHH Leebens-Mack, IJ Leitch, O Maurin, DE Soltis, PS Soltis, GK Wong, WJ Baker, N Wickett. (2019). “A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-medoids Clustering“. Systematic Biology 68(4): 594-606. DOI 10.1093/sysbio/syy086
Performance

Enrich Hundreds of Angiosperm Exons and their Flanking Regions



Fig 1.Heatmap of Gene Recovery Efficiency. Each row is one sample, and each column is one gene. Colors indicate the percentage of the target length (calculated by the mean length of all k-medoid transcripts for each gene) recovered.” (pg 19, Johnson, Pokorny, Dodsworth et al 2019, Syst Bio). Image is Figure 3 from Johnson, Pokorny, Dodsworth et al 2019, Syst Biol.

Fig 2.Total Length of Sequence Recovery for Both Coding and Non-coding Regions Across 353 Loci for 42 Angiosperm Species. Reads were mapped back to either coding sequence (yellow) or coding sequence plus flanking non-coding (i.e. intron) sequence (purple)… The total length of coding sequence targeted was 260,802 bp. The median recovery of coding sequence was 137,046 bp and the median amount of non-coding sequence recovered was 216,816 bp (with at least 8x depth of coverage).” (pg 21, Johnson, Pokorny, Dodsworth et al 2019, Syst Bio). Image is modified version of Figure 4 from Johnson, Pokorny, Dodsworth et al 2019, Syst Bio.


Resources

myBaits Kit Protocol

Current Version: v4
myBaits Manual v4
Visit the myBaits Manual archive page to download past versions.

 

Poster

Poster - PAGXXVII - Angiosperms-353
Title: “A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant”

 

Safety Data Sheet

myBaits Kit SDS
 

Publications

M Johnson, L Pokorny, S Dodsworth, LR Botigue, RS Cowan, A Devault, WL Eiserhardt, N Epitawalage, F Forest, JT Kim, JHH Leebens-Mack, IJ Leitch, O Maurin, DE Soltis, PS Soltis, GK Wong, WJ Baker, N Wickett. (2019). “A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-medoids Clustering“. Systematic Biology 68(4): 594-606. DOI 10.1093/sysbio/syy086

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