myBaits Expert — Predesigned Panels
myBaits Expert – Plant – Angiosperms353
This new panel enriches 100's of single-copy genes orthologous across all angiosperms (flowering plants).

Combining the flexible hybridization power of in-solution target capture with an expertly selected set of orthologous locus sequences, this new probe set has been demonstrated to enrich hundreds of putatively single-copy protein-coding genes across a broad range of angiosperms (flowering plants). Probes were designed from 353 loci, each with 5-15 representative sequences from across all angiosperms which were selected using a novel “k-medoids clustering approach” to maximize taxonomic breadth of the design (Johnson, Pokorny, Dodsworth et al 2018, Systematic Biology).

These probes are broadly applicable for phylogenetic research across all flowering plants, and the kit is available from Daicel Arbor Biosciences as an in-stock catalog kit available for immediate shipment at a low per-reaction cost. As with all myBaits kits, the Angiosperms353 panel is provided as a complete solution target capture kit, including buffers, blockers, and baits, along with an easy-to-use protocol. Or if you would prefer to outsource the work, our myReads NGS service expert scientists are available to perform library preparation, target capture, and sequencing for your entire project.

  • Predesigned Panel – Designed by expert plant geneticists
  • Proven results – Demonstrated utility across broad taxonomic range
  • Freely accessible pipelines – Simple, user-friendly data analysis
  • Cost-effective – Use one bait set across multiple distantly-related taxa

In honor of the latest dual special issues of the American Journal of Botany and Applications in Plant Sciences, devoted to the use of the Angiosperms353 panel for a diverse range of flowering plant genetics research, we are offering a one-time discount on kits and related NGS services for this powerful plant phylogenetics toolkit.

* 10% savings on myBaits Expert Angiosperms353 Kit, including 8, 48, 96 reactions (Promo code: AB_Ang353_Kit_2021_10).

*5% savings on myReads Angiosperms353 NGS project, which could cover entire NGS workflow from DNA extraction through data analysis (Promo code: AB_Ang353_Ser_2021_5). Contact us for project discussion.

The following terms and conditions may apply:
1. The promotion may not apply to regions that use a distributor.
2. The promotion cannot be combined with other discounts.
3. These special discounts are valid on orders placed by October 31, 2021.

Be sure to include the appropriate promotion code when you place an order or contact us for any questions.

Ordering Information
Size
Cat#
Qty
Price
myBaits Expert Angiosperms 353 v1
Kit for enrichment of >350 putatively single-copy nuclear loci from flowering plants (Angiosperms). For phylogenetics.
308108.v5
$1,100
Available in-stock for immediate shipment.


Overview

Enrich Hundreds of Angiosperm Exons and their Flanking Regions

For hundreds of years, scientists have worked to unravel the complex evolutionary origins of the “angiosperms” group of land plants. This enormous, diverse group includes many economically important and ecologically interesting species, and the exact genetic relationships between these groups are still unresolved.

As the power and efficiency of genomic sequencing has increased in recent years, researchers have continued to innovate methods to tackle these major questions. One such method is the Angiosperms353 panel, which uses the flexible, versatile power of NGS hybridization capture to enrich for hundreds of conserved exons believed to be present across the angiosperms group. This universal tool to study exons and their flanking intronic regions allows phylogenomics and population genetics researchers to efficiently focus their sequencing efforts on informative regions that are directly comparable across datasets, for maximum research utility.

NEW! Check out the latest Special Issues on Exploring Angiosperms353: A Universal Toolkit for Flowering Plant Phylogenomics:
Special Issue, American Journal of Botany, Vol 108, Issue 7
Special Issue, Applications in Plant Sciences, Vol 9, Issue 7
Press Release from the Botanical Society of America

 

“Angiosperms sustain the terrestrial living world, including humanity, as primary producers, ecosystem engineers, and earth system regulators.

Angiosperms353 is resetting the phylogenetic baseline with far larger data quantities than have previously been utilized, including the sampling of many genera for which no DNA sequence data are currently available in public repositories.”
Baker et al. (2021)

Performance

A Universal Phylogenomics Solution for Flowering Plants

The myBaits Expert Angiosperms353 kit has been demonstrated to work across a very wide taxonomic and sample range, with excellent exonic and intronic recovery that varies depending on the species and the quality of DNA input. Some example data are shown below, but for the latest and most comprehensive results, check out the most recent Publications that have been published utilizing this kit for an even greater variety of species and sample types.

Fig 1.Heatmap of Gene Recovery Efficiency. Each row is one sample, and each column is one gene. Colors indicate the percentage of the target length (calculated by the mean length of all k-medoid transcripts for each gene) recovered.” (pg 19, Johnson, Pokorny, Dodsworth et al 2019, Syst Bio). Image is Figure 3 from Johnson, Pokorny, Dodsworth et al 2019, Syst Biol.

Fig 2.Total Length of Sequence Recovery for Both Coding and Non-coding Regions Across 353 Loci for 42 Angiosperm Species. Reads were mapped back to either coding sequence (yellow) or coding sequence plus flanking non-coding (i.e. intron) sequence (purple)… The total length of coding sequence targeted was 260,802 bp. The median recovery of coding sequence was 137,046 bp and the median amount of non-coding sequence recovered was 216,816 bp (with at least 8x depth of coverage).” (pg 21, Johnson, Pokorny, Dodsworth et al 2019, Syst Bio). Image is modified version of Figure 4 from Johnson, Pokorny, Dodsworth et al 2019, Syst Bio.
Resources

Angiosperms353 Resources

Original Publication
Johnson, M., Pokorny, L., Dodsworth, S., Botigue, L.R., Cowan, R.S., Devault, A., … Wickett, N. (2019). A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-medoids Clustering. Systematic Biology 68(4): 594-606
GitHub Repository: HybSeq Target Capture of 353 Protein Coding Genes in Any Angiosperm

Related Tools and Reviews
See “Publications” tab for additional papers that utilize the Angiosperms-353 kit.

Poster – PAGXXVII – “A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant”

Kew Tree of Life Explorer: “Building a comprehensive evolutionary tree of life for flowering plants”

PAFTOL (Plant and Fungal Trees of Life) Project: “Discovering and disseminating the evolutionary history of all plant and fungal genera”

 

myBaits Kit Protocol

Current Version: v5

myBaits Manual v5
For kits shipped starting in Sept 2020, with Cat#’s ending in “.v5”

 

Safety Data Sheet

myBaits Kit SDS – US Version – v5 Chemistry – Sept 2020

myBaits Kit SDS – EU Version – v5 Chemistry – Sept 2020

Visit the SDS resources page to download previous versions.
Publications

Featured Publications

The publications listed below highlight some uses of the myBaits® Expert Angiosperms353 hyb capture kit in various plant genetics applications. A more comprehensive searchable database of hundreds of myBaits publications is available on our general Daicel Arbor Biosciences publications page. Thank you to all our new and returning clients for trusting myBaits for your innovative research projects!

For links to hundreds more myBaits publications, visit the publications page!

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New! View the 20 publications from the special issues of the American Journal of Botany and Applications in Plant Sciences,
devoted to the use of the Angiosperms353 panel for a diverse range of flowering plant genetics research.