Sequence the exome of Triticeae and relatives

Acquire deep read coverage at tens to hundreds of megabases of diploid, tetraploid, and hexaploid wheat varieties. Whether for novel variant discovery, detecting introgression, or even routine comprehensive genotyping, the myBaits® Expert Wheat Exome kit reduces sequencing costs by an order of magnitude or more compared to WGS.

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Overview

Developed in collaboration with the International Wheat Genome Sequencing Consortium (IWGSC) using the Chinese Spring RefSeq v1.0 assembly and v1.1 annotation set, this kit tiles 2 million baits across more than 200 Mbp of target space in hexaploid wheat. The dedicated capture protocol was optimized through trial captures on dozens of hexaploid and tetraploid varieties, offering specificity and sensitivity superior to alternatives in the literature.

myBaits kits can be easily integrated into your own research workflows or paired with our customizable Sequencing or Bioinformatics services for a seamless NGS solution. Our lab services experts have processed thousands of wheat and other cereal samples using the myBaits Expert Wheat Exome v1 probe set and are ready to help with yours.

Comprehensive target—Probes target more than 200 Mbp of exonic genome space.

Broad compatibility—The Wheat Exome panel is demonstrated to be compatible with Triticeae, Aegilops, Hordeum, and Secale species, and with any genome ploidy level.

Convenient kits—Each myBaits kit includes probes and hybridization/washing reagents.

Platform agnostic—myBaits kits are able to enrich both short- and long-read NGS libraries, for maximum platform utilization.

Boost your analysis—Couple with the Wheat Regulome V1 panel to sequence promoters and open chromatin.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

Performance

We have evaluated the behavior of this kit across various Triticeae, Aegilops, Secale, and Hordeum. We observed high target specificity and recovery even with distantly related taxa.

The myBaits Expert Wheat Exome panel demonstrates excellent target coverage across a wide range of cultivars. The combination of exonic and non-exonic coverage obtained with the Wheat Exome kit enables it to serve as an effective hexaploid and tetraploid genotyping tool. Even for highly divergent wild emmer wheat (Zavitan), it is easy to achieve high coverage depth at regions of enhanced SNV density.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.