Capture enrichment and massively parallel sequencing for human identification
In the past decade, hybridization capture has gained attention within the forensic field for its possible use in human identification. One of the prim…
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Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
…to reoccurring repeat sequences. Here the authors present CRATES—a modular, one-pot assembly method—and demonstrate the creation of arrays for Cas9, Cas12a and Cas13a for cell-free, bacterial, yeast and mammalian systems….
Phytochrome diversity in green plants and the origin of canonical plant phytochromes
…key life cycle processes, yet their evolutionary origins are not well understood. Using transcriptomic and genomic data, Li et al. find that canonical plant phytochromes originated in a common ances……
Crimean–Congo Hemorrhagic Fever Virus Past Infections Are Associated with Two Innate Immune Response Candidate Genes in Dromedaries
…to humans, such as the Middle East respiratory syndrome coronavirus (MERS-CoV) or Crimean–Congo hemorrhagic fever virus (CCHFV). After several outbreaks of CCHFV in the Arabian Peninsula, recent studies have demonstrated…
Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds
Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and…
Evolutionary biology
…more myBaits® Expert UCE Kits Powerful solution for targeting ultraconserved elements (UCEs) Learn more myBaits® Expert Mito Kits Powerful solution for mitogenome sequencing Learn more Resources Video Webinar Efficient &…
Speciation and gene flow in two sympatric small mammals from Madagascar, Microgale fotsifotsy and M. soricoides (Mammalia: Tenrecidae)
Madagascar’s shrew tenrecs (Mammalia: Tenrecidae; Microgale, Nesogale) represent an excellent system for studying speciation. Most species are endemic to the island’s eastern humid forests, a region renowned for high levels…
Ancient DNA from the extinct South American giant glyptodont Doedicurus sp. (Xenarthra: Glyptodontidae) reveals that glyptodonts evolved from Eocene armadillos
…this study, we used hybridization enrichment and high-throughput sequencing to obtain a partial mitochondrial genome from Doedicurus sp., the largest (1.5 m tall, and 4 m long) and one of…
Genetic evidence for post-glacial expansion from a southern refugium in the eastern moa (Emeus crassus)
…It has been suggested that the extinct eastern moa (Emeus crassus)—a large, flightless bird from New Zealand—behaved analogously during glacial maxima, possibly surviving only in a restricted area of lowland…
Evolutionary history of enigmatic bears in the Tibetan Plateau–Himalaya region and the identity of the yeti
…be local, extant bears. Complete mitochondrial genomes were assembled for Himalayan brown bear (U. a. isabellinus) and black bear (U. t. laniger) for the first time. Our results demonstrate that…
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