Targeted Sequencing

Next-generation sequencing (NGS) platforms have revolutionized the scale and power of modern genetics studies.

Targeted sequencing technology can increase the scale of projects and maintain relatively low cost by focusing sequencing efforts on specific regions of interest in samples, facilitating orders of magnitude more efficient NGS. In-solution target capture, also known as target enrichment or sequence capture, utilizes pools of thousands of long custom biotinylated oligonucleotides to selectively enrich for specific regions of interest in an NGS library prior to sequencing. The technological development to manufacture affordable complex oligo pools, such as those provided by Arbor Biosciences, enables this enrichment technique to be feasible for projects of any scale, ranging from just one to up to tens of thousands of loci captured simultaneously.

Unlike amplicon-based panels or other genotyping technologies such as SNP arrays, genotyping by sequencing (GBS) or RAD-seq, in-solution targeted sequencing can be used to assess any type of genetic feature appropriate for NGS, such as point mutations, copy number variation (CNV), small and large indels, and more. In addition, target capture can be used on any type of specimen, even samples with short, degraded target molecules such as archaeological, forensic, or cell-free DNA. Target capture is compatible with all major NGS platforms (Illumina®, Ion Torrent®, PacBio®, and Nanopore®), and the same bait set can be used for both short and long-read sequencing.

The use of RNA probes for target capture provides a strong RNA-DNA bond, allowing for highly specific yet flexible enrichment of target molecules, even from extremely complex samples. myBaits Custom Kits tailored for specific target regions are our most popular choice, allowing enrichment for specific SNPs, exons, genes, and other sequence motifs from genomic or metagenomic samples. Arbor Biosciences also offers a variety of predesigned kit options for specific research applications, such as mitochondrial DNA sequencing, ultraconserved element sequencing, whole-genome enrichment from metagenomic samples, and oncology research.

Featured Publications

O.A. Ali et al. (2017). RAD Capture (Rapture): Flexible and Efficient Sequence-Based Genotyping. Genetics

M. Giolai et al. (2017). Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire. BMC Genomics

J. Starrett et al. (2017). High phylogenetic utility of an ultraconserved element probe set designed for Arachnida. Molecular Ecology Resources

J.M. Enk et al. (2016). Mammuthus Population Dynamics in Late Pleistocene North America: Divergence, Phylogeography, and Introgression. Frontiers in Ecology and Evolution

J.A. Tennessen et al. (2017). A Targeted Capture Linkage Map Anchors the Genome of the Schistosomiasis Vector Snail, Biomphalaria glabrata. Genes Genomes Genetics

S. Singhal et al. (2017). Squamate Conserved Loci (SqCL): A unified set of conserved loci for phylogenomics and population genetics of squamate reptiles. Molecular Ecology Resources

B.M. vonHoldt, E. Shuldiner, et al. (2017). Structural variants in genes associated with human Williams-Beuren syndrome underlie stereotypical hypersociability in domestic dogs. Science Advances

J. Trimpert et al. (2017). A phylogenomic analysis of Marek’s disease virus reveals independent paths to virulence in Eurasia and North America. Evolutionary Applications

C. Li et al. (2017). Capturing protein-coding genes across highly divergent species. BioTechniques

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