Yes, scaling up the volume is possible, but above 50 uL we recommend shaking and/or switching to a reaction vessel with higher surface:volume ratio to allow proper oxygenation of the reaction mix. myTXTL reactions are very sensitive to the amount of dissolved oxygen. If using over 50 uL volume, a flat-bottomed ELISA plate or tissue culture plate may be advised along with shaking. We advise testing such a setup with our positive control deGFP plasmids or linear fragments that come with the kit. The key is to balance oxygenation and avoiding the reactions drying out due to too much surface area.