Steps of myTags Custom production using the Indexed Synthesis technique:

1. Probes are designed for each target region separately

2. All probe sequences (up to 27K total) are synthesized together

3. Using unique indexes, each individual probeset is separated into individual oligo pools via PCR deconvolution

4. Composition of oligo pools is verified with next-generation sequencing (NGS)

Note: Short index sequences remain present on the final probe oligos, but are short and not expected to interfere with downstream (F)ISH protocols.