Yes! Parameters that influence protein production efficiency are:

  • Gene cassette construction (promoter strength, position of affinity tag, TXTL elements)
  • DNA purity
  • DNA concentration
  • Incubation temperature, time, and vessel
  • Presence of folding helpers, chaperones, oxidizing agents

and should therefore be evaluated for optimization. Please also see our recommendations on Template Design in the current myTXTL handbook.

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