What device do you recommend for incubating myTXTL reactions – incubator, thermoblock or water bath?

Due to the small reaction volume of 12 μL, it is very important to avoid condensation of water on the lid of the reaction tube, as it considerably increases the concentration of myTXTL reaction components. This can lead to poor or irreproducible kit performance. If possible, incubation in an incubator and water bath/Armor beads is best. Water facilitates a faster heat transfer than air and a water bath shows low temperature fluctuation, which should – combined with a closed environment with constant temperature surrounding the entire tube – lead to higher reproducibility and yield. A PCR thermoblock did not perform well with tubes or a multiwell plate.