Several factors can hinder protein synthesis in the myTXTL cell-free protein expression system, including:

  1. Master mix inactivation due to improper storage. The myTXTL master mix must be stored at -80°C and number of freeze-thaw cycles should be minimized.
  2. Improper reaction setup, Please review the recommendations to set up a myTXTL reaction in the current myTXTL manual.
  3. Contamination of myTXTL reaction with nuclease. To avoid nuclease contamination, wear gloves and use nuclease-free water, sterilized tips and tubes. Use deionized, nuclease-free, “molecular grade” water for myTXTL reaction setup.
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