Name: myReads – NGS Services
Brand: Arbor Biosciences

NGS Services

myReads – NGS Services

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The myReads NGS services team at Daicel Arbor Biosciences is ready for your next sequencing project.

Next Generation Sequencing – NGS Services

We offer competitively-priced services for DNA- or RNA-seq library preparation, targeted sequencing via hybridization-based capture with myBaits, and next-generation sequencing (NGS) on multiple platforms. In collaboration with you, we assist in selecting the most appropriate project configuration to accommodate the research goals, sample quality, and budget of your project. We accept NGS projects from any organism or application, and routinely utilize one of our myBaits target capture panels, whether for a customized set of targets or one of our many predesigned panels.

Proven system – Consistent results from high-complexity NGS library preparation
Fast turn-around – From DNA to sequence data in weeks
Multiple sample types – Utilize fresh, degraded, metagenomics, or even ancient DNA
Collaborative process – Design consultation to meet project budget and goals
Multiple sequencing platforms – Select from Illumina, Ion Torrent, PacBio
Volume discounts – Tiered per-sample pricing for larger projects

New! We now offer a range of Service Packages including library prep, hybridization capture, and sequencing for different project types, such as Standard DNA, Degraded DNA, Long-Insert DNA, and more. See below to learn more about our packages or contact us today to get started on your next project.

Timeline

A typical custom myReads project takes 1-4 months, depending on current queues. This includes time for new myBaits Custom kit probe design and synthesis (if applicable), sample QC, NGS library preparation, hybridization capture, and Illumina sequencing with our standard configuration.

Pricing

We offer affordable pricing for projects of any size, and can typically provide project estimates within 24-48 hours. Relevant parameters include (a) number of samples, (b) DNA/RNA amount, damage, and purity, (c) hybridization capture target and experimental details, (d) sequencing platform and depth, and more.

Overview
Packages
Resources
FAQs
Publications

Overview

Introduction

The myReads NGS Services team at Daicel Arbor Biosciences provide full-service solutions for NGS shotgun or targeted sequencing projects, for both DNA and RNA sequencing. This continues Daicel Arbor Biosciences long tradition of offering customized, affordable targeted sequencing solutions to the NGS research community, especially for complex or difficult samples. The myReads seasoned scientific team has decades of collective experience with planning and executing custom NGS projects, including a wide range of novel applications and difficult specimens such as ancient DNA. Our team has facilitated hundreds of projects and thousands of client samples through successful library preparation, capture, and/or sequencing. Contact us today to jump start your next NGS project!

NGS Library Preparation

Short-insert DNA or RNA library prep
Long-insert DNA or cDNA library prep
Specialized double or single-stranded chemistry for degraded/ancient DNA

Target Enrichment (myBaits)

New myBaits Custom panels with free design assistance
Predesigned myBaits Expert panels for special applications

Short & Long Read Sequencing

Illumina^® NovaSeq and MiSeq
Ion Torrent^® Proton
PacBio^® Sequel II
Oxford Nanopore Technologies^®

To initiate a project or obtain an estimate, please contact us with details on the number of samples, quality of your samples’ DNA or RNA, and a description of your overall research goals. Our scientific experts can recommend appropriate parameters for any project and accommodate most specific preferences for configuration options such as library construction methods, capture conditions, and sequencing parameters to maximize data output from the project. Learn more below about the myReads process and how to submit a project proposal.

What We Do

Project and probe design consultation
Library prep, hybridization capture, and sequencing on Illumina, PacBio, or Ion Torrent platforms
Specialists in targeted sequencing

How It Works

Contact the experienced myReads scientists for customized project conferral and estimate
Provide sequences for custom myBaits design (if applicable)
Ship us DNA or RNA samples, following our sample preparation and submission guidelines
Receive back either enriched NGS libraries or digital NGS FASTQ read data

Why Choose myReads?

Competitive pricing
Dedicated scientific team
Experience working with a broad spectrum of sample types (e.g. fresh, degraded, metagenomic, ancient)
Proven results
Experience in many specialized applications:
- Genotyping, marker resequencing, marker discovery
- Variant discovery in non-model organisms
- Herbarium, ancient, and museum DNA sequencing
- Phylogenetically-informative region sequencing (e.g. UCEs)
- Disease resistance gene sequencing (RenSeq)
- Long-insert targeted sequencing for PacBio and Oxford Nanopore
- Target enrichment for methylation analysis

Packages

NGS Service Packages

Our full service packages including NGS library preparation, hybridization capture, and sequencing with flexible, customizable options for different sample and project types! Pick a package that fits your project needs, or mix and match. If you don’t see something that works for you, we offer a wide range of à la carte services that can be customized to meet your needs. See packages and policies in the resources tab.

Standard DNA for freshly-extracted, good quality DNA

Includes QC, fragmentation, library preparation, 8-12-plex captures, 1Gbp of PE150 sequencing per sample
All samples must be high-molecular weight (>10kbp modal length), high quality (UV 260:280 1.7-1.9), and high mass (>1 μg DNA)
Library preparation performance commitment for samples meeting our guidelines (see Service Policies for more info)
Complementary QC report allowing you to make decision

Degraded DNA for older specimens, herbarium material, or samples of unknown quality

Includes extended QC, fragmentation (where necessary), library preparation with specialized chemistry (where necessary), 4-6-plex captures, 1Gbp of PE150 sequencing per sample
Samples may have a range of molecular weights, UV 260:280 values, and mass (>10 ng DNA)
Performance is not guaranteed for degraded samples (see Service Policies for more info)
Complementary QC report allowing you to make decisions about which samples to keep, drop, or supplement

Ancient DNA Double-Stranded for museum, historical, or ancient DNA from other sources

Includes QC, double-stranded library preparation with specialized chemistry (the “gold standard” for ancient DNA), 2-4-plex dual-round captures, 1Gbp of PE150 sequencing per sample
All samples must be low-molecular weight (<1kbp modal length)
Performance is not guaranteed for ancient samples (see Service Policies for more info)
Complementary QC report allowing you to make decisions about which samples to keep, drop, or supplement

Ancient DNA Single-Stranded for museum, historical, or ancient DNA, cfDNA, or single-stranded cDNA

Includes QC, single-stranded library preparation with specialized chemistry, 2-4-plex dual-round captures, 1Gbp of PE150 sequencing per sample
All samples must be low-molecular weight (<1kbp modal length)
Performance is not guaranteed for ancient samples (see Service Policies for more info)
Complementary QC report allowing you to make decisions about which samples to keep, drop, or supplement

Long-insert DNA for DNA or cDNA intended for PacBio sequencing

Includes QC, long-insert precursor library preparation, 3-plex captures, 24 samples per SMRTbell preparation & Sequel II cell
All samples must be high-molecular weight (>10kbp modal length), high quality (UV 260:280 1.7-1.9), and high mass (>5 μg DNA)
Library preparation performance commitment for samples meeting our guidelines (see Service Policies for more info)
Complementary QC report allowing you to make decisions about which samples to keep, drop, or supplement

Standard RNA for high quality RNA or mRNA

Includes QC, DNAase treatment, purification, 8-12-plex captures, 1 Gbp of PE150 sequencing per sample
All samples must be high quality (UV 260:280 1.9-2.1), and high mass (>250nμg DNA)
Additional options for mRNA capture & rRNA depletion from human/mouse/rat samples
Library preparation performance commitment for samples meeting our guidelines (see Service Policies for more info)
Complementary QC report allowing you to make decisions about which samples to keep, drop, or supplement

If you have special needs for your NGS project, such as additional sequencing or a non-hybridization capture project, we also offer a wide range of à la carte services that can be customized to meet your needs.

Resources

Guidelines and Forms

myReads NGS Service Policies
myReads Sample Preparation Guide
myReads DNA Sample Submission Form
myReads RNA Sample Submission Form
myReads Library Sample Submission Form
myReads Plant Tissue Sample Submission Form
myReads Ancient Tissue Sample Submission Form

Information Sheets

myReads NGS Services – Product Sheet
myReads NGS Service Packages
myReads Wheat Targeted Sequencing Packages

FAQs

Why should I choose Daicel Arbor Biosciences for my NGS service?

Our goal is to provide NGS service for your project as if you were working directly with one of your research collaborators. We strive for a transparent and cooperative approach to NGS projects, and will communicate openly with you to find the right solution for your individual research design needs. We have decades of collective experience in molecular biology, including target capture & high-throughput sequencing, and working with difficult specimens.

In order to facilitate transparent, collaborative projects, we have opted to not offer specific guarantees for target capture & sequencing service projects, since every custom project is completely unique. In science, sometimes research projects do not go 100% according to plan. However, you will only pay for the services that we actually perform. We will give every project our individual attention, and all laboratory work is performed by hand on the bench by our team of dedicated & experienced researchers. Our goal is to provide you with the very same dedicated laboratory attention that you & your research team would give to your own work. You can be assured that we will communicate openly with you about all aspects of your project, and will not commit to a project before first discussing all the options with you.

What services do you offer?

We offer a full suite of services for next-generation sequencing projects, on both regular specimens as well as degraded specimens such ancient DNA including:

Library preparation for Ion Torrent, Illumina, and PacBio
Target enrichment using myBaits catalog and custom kits
High-throughput sequencing on Ion Torrent, Illumina, and PacBio instruments

Fig 1. Demonstration of a range of customizable NGS library insert size options available from myReads NGS services. Daicel Arbor Biosciences can help you to choose the best average insert length appropriate for your specific myBaits target capture project, whether SNP resequencing, exon capture, UCE sequencing, or more. Depending on the quality of your DNA, the nature of your desired target regions, and your planned sequencing platform and read protocol, your project may benefit from either shorter or longer NGS library sizes.

What are your sample submission requirements?

The current submission guidelines and instructions are available under the “Resources” tab of this webpage. These guidelines should be sufficient for most standard DNA or RNA extracts derived from fresh, high-quality, relatively recent specimens (e.g. DNA extracted from fresh, frozen, or alcohol-preserved tissues).

In addition, we are currently accepting most service projects for atypical or sensitive samples, including specimens with degraded or rare targets (e.g., ancient DNA, museum, archival, metagenomic, environmental, etc). However, you should first contact us with more information about your samples & research goals, so that we can determine if we are able to accept your project. We may provide you with a customized set of sample submission requirements that are tailored for your particular application.

Can you work with smaller amounts of DNA/RNA than the requested amount?

Yes we can, however we caution that there is a higher risk of sample and/or target drop-out for low-input and/or low-quality samples. In our experience across many project types, the recommended amounts outlined in the Submission Guidelines provide the best results.

What is the minimum number of samples that I can include in a myReads project?

We do not have a minimum sample number, but we advise that smaller projects will have a higher per-sample cost than larger projects due to the labor required to process a batch of samples and our volume discount pricing.

What turnaround time & coverage depth guarantees do you offer?

Due to the inherently variable nature of target capture & high-throughput sequencing experiments (especially for new, untested, custom baitsets), we are unable to offer service guarantees related to turnaround time, unique read sequencing coverage depth, on-target percentage, and other experimental outcomes.

In order to facilitate transparent, collaborative projects, we have opted to not offer specific guarantees for service projects, since every custom project is completely unique. In science, sometimes research projects do not go 100% according to plan, and for target capture, it is very difficult to plan for specific sequencing outcomes without prior experimental data. However, you will only pay for the services that we actually perform, and we will not accept a project before discussing all the options with you.

You will receive updates and estimated timelines for completion of different stages of your project. If you are working within a specific deadline, we will certainly make every effort to accommodate that, though we cannot guarantee that it will be possible to finish your project within that window.

Can I send you my libraries for target capture?

We would prefer that we make the libraries so that we have direct control over the uniformity and quality of the libraries going into target capture. However, yes, you can provide us directly with libraries that you make yourself. Please confer with us to make sure that we are fully aware of the type of libraries that you are using, since it is critical to choose the correct adapter-specific blocking oligos during capture and the right amplification primers after capture.

Can I use multiple different baitsets for capture service?

Yes, absolutely. We typically have enough material to perform multiple different types of captures from a single set of libraries that we generate. Please contact us for more information about pricing for your individual project configuration needs.

Do you offer DNA extraction services?

We currently do not offer DNA extraction as a standard service option. However, we do have the facilities and expertise for performing DNA extraction on both regular and degraded specimens. Therefore, we may be able to offer this for your project, depending on the circumstances. Please contact us directly to inquire about special service options.

What type of DNA do you accept for service (human, plant, animal, degraded, etc)?

We offer NGS service on any type of non-hazardous DNA extract, whether from high-quality or degraded specimens. However, your samples should still meet our standard submission requirements for your type of service project, including relevant negative controls where applicable. Otherwise, your samples may be unable to pass our quality controls, or your project could be delayed. Please note that currently we are not accepting projects on human ancient DNA specimens for target capture of human DNA.

Publications

Featured myReads Publications

We are proud to highlight these high-quality publications from researchers around the world! All of these publications included some or all stages of NGS library preparation, myBaits enrichment, and/or sequencing that were performed and/or arranged by the myReads team. Thank you for allowing us to assist with these fascinating projects!