iGEM 2020 Grant Sponsorship
The International Genetically Engineered Machine (iGEM) competition is the largest competition of students in the field of synthetic biology. Every Spring since 2004, interdisciplinary teams from all over the world…
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Impact of Porous Matrices and Concentration by Lyophilization on Cell-Free Expression
Cell-free expression systems have drawn increasing attention as a tool to achieve complex biological functions outside of the cell. Several applications of the technology involve the delivery of functionality to…
Characterization of the all-E. coli transcription translation system myTXTL by mass spectrometry
Rationale Cell-free transcription-translation (TXTL) is becoming a popular technology to prototype and engineer biological systems outside living organisms. TXTL relies commonly to a cytoplasmic extract that provides the molecular components…
Wallacean and Melanesian Islands Promote Higher Rates of Diversification within the Global Passerine Radiation Corvides
Abstract The complex island archipelagoes of Wallacea and Melanesia have provided empirical data behind integral theories in evolutionary biology, including allopatric speciation and island biogeography. Yet, questions regarding the relative…
Exon capture optimization in amphibians with large genomes
Gathering genomic-scale data efficiently is challenging for nonmodel species with large, complex genomes. Transcriptome sequencing is accessible for organisms with large genomes, and sequence capture probes can be designed from…
Mammal mitogenomics from invertebrate-derived DNA
…complete mammalian mitogenomes and therefore bring the field closer to population-level analyses. Specifically, we used mitogenomic hybridization capture coupled with high-throughput sequencing to analyze individual (N = 7) or pooled…
Phylogeny of Crataegus (Rosaceae) based on 257 nuclear loci and chloroplast genomes: evaluating the impact of hybridization
…compared individual gene trees with each other, and with both the maximum likelihood plastome tree and the nuclear concatenated and multilocus coalescent-based trees. Tree comparisons provided a promising, if challenging…
Gene expression inside liposomes: from early studies to current protocols (review).
…cells. At this aim, four currently employed protocols are compared and commented, with major emphasis on the droplet transfer method, which is attractive due to its simplicity and encapsulation efficiency….
UCE Phylogenomics Resolves Major Relationships Among Ectaheteromorph Ants (Hymenoptera: Formicidae: Ectatomminae, Heteroponerinae): A New Classification For the Subfamilies and the Description of a New Genus
…includes the subfamily Heteroponerinae new synonym and its single tribe Heteroponerini new combination. Eleven monophyletic groups are recognized as genera: Acanthoponera, Alfaria status revived, Boltonia Camacho and Feitosa new genus,…
Do you have recommendations on how to prepare the plasmid template for the myTXTL reaction?
…plasmid DNA with standard commercial kits usually involves sample treatment with RNase, which may not be completely removed during downstream processing. Thus, we strongly recommend subjecting the prepared DNA to…
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