Some tropical plant families, such as the Sapotaceae, have a complex taxonomy, which can be resolved using Next Generation Sequencing (NGS). For most groups however, methodological protocols are still missing….
…vaccinated with a single dose of ChAdOx1 nCoV-19. Here, we observe a 9.5-fold reduction of virus neutralizing antibody titer in vaccinated hamster sera against B.1.351 compared to B.1.1.7. Vaccinated hamsters…
Abstract The complex island archipelagoes of Wallacea and Melanesia have provided empirical data behind integral theories in evolutionary biology, including allopatric speciation and island biogeography. Yet, questions regarding the relative…
…complete mammalian mitogenomes and therefore bring the field closer to population-level analyses. Specifically, we used mitogenomic hybridization capture coupled with high-throughput sequencing to analyze individual (N = 7) or pooled…
…includes the subfamily Heteroponerinae new synonym and its single tribe Heteroponerini new combination. Eleven monophyletic groups are recognized as genera: Acanthoponera, Alfaria status revived, Boltonia Camacho and Feitosa new genus,…
…plasmid DNA with standard commercial kits usually involves sample treatment with RNase, which may not be completely removed during downstream processing. Thus, we strongly recommend subjecting the prepared DNA to…
Rationale Cell-free transcription-translation (TXTL) is becoming a popular technology to prototype and engineer biological systems outside living organisms. TXTL relies commonly to a cytoplasmic extract that provides the molecular components…
…cells. At this aim, four currently employed protocols are compared and commented, with major emphasis on the droplet transfer method, which is attractive due to its simplicity and encapsulation efficiency….
The International Genetically Engineered Machine (iGEM) competition is the largest competition of students in the field of synthetic biology. Every Spring since 2004, interdisciplinary teams from all over the world…
…compared individual gene trees with each other, and with both the maximum likelihood plastome tree and the nuclear concatenated and multilocus coalescent-based trees. Tree comparisons provided a promising, if challenging…
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